Multivariate modelling with 1H NMR of pleural effusion in murine cerebral malaria

Malaria Journal

Research

Shobhona Sharma¹  Arjun Sengupta²  Soumita Ghosh²  Haripalsingh M Sonawat² 

[1] Department of Biological Sciences, Tata Institute of Fundamental Research, 1-Homi Bhabha Road, 400 005, Mumbai, India;Department of Chemical Sciences, Tata Institute of Fundamental Research, 1-Homi Bhabha Road, Mumbai-400005, Mumbai, India;

关键词: Cerebral Malaria;    Plasmodium berghei;    pleural effusion;    NMR;    Orthogonal Partial Least Square Discriminant Analysis;    Multiway Principal Component Analysis;    Multivariate Curve Resolution;   

DOI  :  10.1186/1475-2875-10-330

 received in 2011-08-19, accepted in 2011-11-02,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCerebral malaria is a clinical manifestation of Plasmodium falciparum infection. Although brain damage is the predominant pathophysiological complication of cerebral malaria (CM), respiratory distress, acute lung injury, hydrothorax/pleural effusion are also observed in several cases. Immunological parameters have been assessed in pleural fluid in murine models; however there are no reports of characterization of metabolites present in pleural effusion.Methods1H NMR of the sera and the pleural effusion of cerebral malaria infected mice were analyzed using principal component analysis, orthogonal partial least square analysis, multiway principal component analysis, and multivariate curve resolution.ResultsIt has been observed that there was 100% occurrence of pleural effusion (PE) in the mice affected with CM, as opposed to those are non-cerebral and succumbing to hyperparasitaemia (NCM/HP). An analysis of 1H NMR and SDS-PAGE profile of PE and serum samples of each of the CM mice exhibited a similar profile in terms of constituents. Multivariate analysis on these two classes of biofluids was performed and significant differences were detected in concentrations of metabolites. Glucose, creatine and glutamine contents were high in the PE and lipids being high in the sera. Multivariate curve resolution between sera and pleural effusion showed that changes in PE co-varied with that of serum in CM mice. The increase of glucose in PE is negatively correlated to the glucose in serum in CM as obtained from the result of multiway principal component analysis.ConclusionsThis study reports for the first time, the characterization of metabolites in pleural effusion formed during murine cerebral malaria. The study indicates that the origin of PE metabolites in murine CM may be the serum. The loss of the components like glucose, glutamine and creatine into the PE may worsen the situation of patients, in conjunction with the enhanced glycolysis, glutaminolysis and increased activity of creatine phophokinase which are already reported characteristic pathophysiological features of malaria.

【 授权许可】

Unknown   
© Ghosh et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.