Upregulation of BMP1 through ncRNAs correlates with adverse outcomes and immune infiltration in clear cell renal cell carcinoma

European Journal of Medical Research

Research

Wenjing Dong¹  Tianxin Lin²  Shaopeng Qiu³  Jinquan Luo⁴  Shengxing Feng⁴  Runqiang Yuan⁴  Mancheng Gong⁴  Dongsheng Zhou⁴ 

[1] Department of Oncology, The People’s Hospital of Zhongshan, No. 2 Sunwen East Road, 528403, Zhongshan, Guangdong, China;Department of Urology, Sun Yat-sen Memorial Hospital, 510080, Guangzhou, Guangdong, China;Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China;Department of Urology, The People’s Hospital of Zhongshan, 528403, Zhongshan, Guangdong, China;

关键词: BMP1;    ccRCC;    Prognosis;    Survival;    Immune cells;   

DOI  :  10.1186/s40001-023-01422-x

 received in 2023-06-29, accepted in 2023-10-01,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundRenal cell carcinoma (RCC) accounts for approximately 2–3% of all adult malignancies. Clear cell renal cell carcinoma (ccRCC), which comprises 70–80% of all RCC cases, is the most common histological subtype.MethodsccRCC transcriptome data and clinical information were downloaded from the TCGA database. We used the TCGA and GEPIA databases to analyze relative expression of BMP1 in various types of human cancer. GEPIA was used to perform survival analysis for BMP1 in various cancer types. Upstream binding miRNAs of BMP1 were obtained through several important target gene prediction tools. StarBase was used to predict candidate miRNAs that may bind to BMP1 and candidate lncRNAs that may bind to hsa-miR-532-3p. We analyzed the association between expression of BMP1 and immune cell infiltration levels in ccRCC using the TIMER website. The relationship between BMP1 expression levels and immune checkpoint expression levels was also investigated.ResultsBMP1 was upregulated in GBM, HNSC, KIRC, KIRP and STAD and downregulated in KICH and PRAD. Combined with OS and DFS, BMP1 can be used as a biomarker for poor prognosis among patients with KIRC. Through expression analysis, survival analysis and correlation analysis, LINC00685, SLC16A1-AS1, PVT1, VPS9D1-AS1, SNHG15 and the CCDC18-AS1/hsa-miR-532-3p/BMP1 axis were established as the most potential upstream ncRNA-related pathways of BMP1 in ccRCC. Furthermore, we found that BMP1 levels correlated significantly positively with tumor immune cell infiltration, biomarkers of immune cells, and immune checkpoint expression.ConclusionOur results demonstrate that ncRNA-mediated high expression of BMP1 is associated with poor prognosis and tumor immune infiltration in ccRCC.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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