PVM/MA-shelled selol nanocapsules promote cell cycle arrest in A549 lung adenocarcinoma cells

Journal of Nanobiotechnology

Research

África González-Fernández¹  Rosana Simón-Vázquez¹  Piotr Suchocki²  Mayara Simonelly Costa dos Santos³  Ludmilla Regina de Souza³  Sônia Nair Báo⁴  Ricardo Bentes Azevedo⁴  Luis Alexandre Muehlmann⁴  Rayane Ganassin⁴  Graziella Anselmo Joanitti⁴  Ewa Mosiniewicz-Szablewska⁵  Paulo César Morais⁶ 

[1] Biomedical Research Center (CINBIO), Institute of Biomedical Research of Vigo, University of Vigo, 36310, Vigo, Pontevedra, Spain;Department of Bioanalysis and Drugs Analysis, Warsaw Medical University, 02-097, Warsaw, Poland;Department of Pharmaceutical Chemistry, National Medicines Institute, 00-725, Warsaw, Poland;Institute of Biological Sciences, Molecular Biology Programme, University of Brasília, 70910-900, Brasília, DF, Brazil;Institute of Biological Sciences, University of Brasília, 70910-900, Brasília, DF, Brazil;Institute of Physics, Polish Academy of Sciences, 02-668, Warsaw, Poland;Institute of Physics, University of Brasília, 70910-900, Brasília, DF, Brazil;School of Automation, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, China;

关键词: A549 Cell;    Propidium Iodide;    Selenite;    Mitochondrial Membrane Potential;    HL60 Cell;   

DOI  :  10.1186/s12951-014-0032-x

 received in 2014-06-24, accepted in 2014-08-12,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSelol is an oily mixture of selenitetriacylglycerides that was obtained as a semi-synthetic compound containing selenite. Selol is effective against cancerous cells and less toxic to normal cells compared with inorganic forms of selenite. However, Selol’s hydrophobicity hinders its administration in vivo. Therefore, the present study aimed to produce a formulation of Selol nanocapsules (SPN) and to test its effectiveness against pulmonary adenocarcinoma cells (A549).ResultsNanocapsules were produced through an interfacial nanoprecipitation method. The polymer shell was composed of poly(methyl vinyl ether-co-maleic anhydride) (PVM/MA) copolymer. The obtained nanocapsules were monodisperse and stable. Both free Selol (S) and SPN reduced the viability of A549 cells, whereas S induced a greater reduction in non-tumor cell viability than SPN. The suppressor effect of SPN was primarily associated to the G2/M arrest of the cell cycle, as was corroborated by the down-regulations of the CCNB1 and CDC25C genes. Apoptosis and necrosis were induced by Selol in a discrete percentage of A549 cells. SPN also increased the production of reactive oxygen species, leading to oxidative cellular damage and to the overexpression of the GPX1, CYP1A1, BAX and BCL2 genes.ConclusionsThis study presents a stable formulation of PVM/MA-shelled Selol nanocapsules and provides the first demonstration that Selol promotes G2/M arrest in cancerous cells.

【 授权许可】

Unknown   
© de Souza et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.