A review on Lactococcus lactis: from food to factory

Microbial Cell Factories

Review

Raha Abdul Rahim¹  Adelene Ai-Lian Song²  Lionel L. A. In³  Swee Hua Erin Lim⁴ 

[1] Department of Cell & Molecular Biology, Faculty of Biotechnology & Biomolecular Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia;Department of Microbiology, Faculty of Biotechnology & Biomolecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Functional Food Research Group, Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia;Perdana University-Royal College of Surgeons in Ireland, Perdana University, Block B and D, MAEPS Building, MARDI Complex, Jalan MAEPS Perdana, 43400, Serdang, Selangor, Malaysia;

关键词: Lactococcus lactis;    Heterologous proteins;    Recombinant proteins;    Expression systems;    Secretion;    Surface display;    Microbial cell factory;   

DOI  :  10.1186/s12934-017-0669-x

 received in 2016-11-13, accepted in 2017-03-28,  发布年份 2017

来源: Springer

【 摘 要 】

Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features of this gram-positive lactic acid non-colonizing gut bacteria include its generally recognized as safe (GRAS) status, probiotic properties, the absence of inclusion bodies and endotoxins, surface display and extracellular secretion technology, and a diverse selection of cloning and inducible expression vectors. This have made L. lactis a desirable and promising host on par with other well established model bacterial or yeast systems such as Escherichia coli, Salmonella cerevisiae and Bacillus subtilis. In this article, we review recent technological advancements, challenges, future prospects and current diversified examples on the use of L. lactis as a microbial cell factory. Additionally, we will also highlight latest medical-based applications involving whole-cell L. lactis as a live delivery vector for the administration of therapeutics against both communicable and non-communicable diseases.

【 授权许可】

CC BY   
© The Author(s) 2017