Characterization of various cell lines from different ampullary cancer subtypes and cancer associated fibroblast-mediated responses

BMC Cancer

Research Article

Frank Makowiec¹  Ulrich Theodor Hopt²  Tobias Keck³  Dirk Bausch³  Louisa Bolm³  Ulrich Friedrich Wellner⁴  Hannah Fuellgraf⁵  Peter Bronsert⁶  Martin Werner⁶  Claudio Sorio⁷  Aldo Scarpa⁷  Martin L. Biniossek⁸  Zon Weng Lai⁸  Oliver Schilling⁹ 

[1] Clinic for General and Visceral Surgery, University Medical Center Freiburg, Freiburg, Germany;Clinic for General and Visceral Surgery, University Medical Center Freiburg, Freiburg, Germany;Klinik für Chirurgie, Ratzeburger Allee 160, 23562, Lübeck, Germany;Clinic for Surgery, UKSH Campus Lübeck, Lübeck, Germany;Clinic for Surgery, UKSH Campus Lübeck, Lübeck, Germany;Comprehensive Cancer Center Freiburg, Freiburg, Germany;Klinik für Chirurgie, Ratzeburger Allee 160, 23562, Lübeck, Germany;Department of Pathology, University Medical Center Freiburg, Freiburg, Germany;Department of Pathology, University Medical Center Freiburg, Freiburg, Germany;German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), D-69120, Heidelberg, Germany;Comprehensive Cancer Center Freiburg, Freiburg, Germany;Dipartimento di Patologia, Universita di Verona, Verona, Italy;Institute of Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany;Institute of Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany;BIOSS Centre for Biological Signaling Studies, University of Freiburg, Freiburg, Germany;German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), D-69120, Heidelberg, Germany;

关键词: Ampullary adenocarcinoma;    Fibroblast;    Differentiation;    Intestinal;    Pancreatobiliary;    Dysplastic;    Cell proliferation;    Cell invasion;   

DOI  :  10.1186/s12885-016-2193-5

 received in 2015-08-27, accepted in 2016-02-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAmpullary cancer is a relatively rare form of cancer and usually treated by pancreatoduodenectomy, followed by adjuvant therapy. The intestinal subtype is associated with markedly improved prognosis after resection. At present, only few cell lines are available for in vitro studies of ampullary cancer and they have not been collectively characterized.MethodsWe characterize five ampullary cancer cell lines by subtype maker expression, epithelial-mesenchymal transition (EMT) features, growth and invasion, drug sensitivity and response to cancer-associated fibroblast conditioned medium (CAF-CM).ResultsOn the basis of EMT features, subtype marker expression, growth, invasion and drug sensitivity three types of cell lines could be distinguished: mesenchymal-like, pancreatobiliary-like and intestinal-like. Heterogeneous effects from the cell lines in response to CAF-CM, such as different growth rates, induction of EMT markers as well as suppression of intestinal differentiation markers were observed. In addition, proteomic analysis showed a clear difference in intestinal-like cell line from other cell lines.ConclusionMost of the available AMPAC cell lines seem to reflect a poorly differentiated pancreatobiliary or mesenchymal-like phenotype, which is consistent to their origin. We suggest that the most appropriate cell line model for intestinal-like AMPAC is the SNU869, while others seem to reflect aggressive AMPAC subtypes.

【 授权许可】

CC BY   
© Lai et al. 2016