An adeno-associated viral vector transduces the rat hypothalamus and amygdala more efficient than a lentiviral vector

BMC Neuroscience

Methodology Article

Erno Vreugdenhil¹  Carlos P Fitzsimons¹  Keith M Garner²  Maike AD Brans²  Mieneke CM Luijendijk²  Roger AH Adan²  Marijke WA de Backer² 

[1] Medical Pharmacology Department, Leiden/Amsterdam Center for Drug Research, Leiden University Medical Center, Leiden University, Leiden, the Netherlands;Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Centre Utrecht, Utrecht, the Netherlands;

关键词: Green Fluorescent Protein;    Lateral Hypothalamus;    Transduction Efficiency;    Green Fluorescent Protein Gene;    AAV1 Vector;   

DOI  :  10.1186/1471-2202-11-81

 received in 2010-02-26, accepted in 2010-07-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThis study compared the transduction efficiencies of an adeno-associated viral (AAV) vector, which was pseudotyped with an AAV1 capsid and encoded the green fluorescent protein (GFP), with a lentiviral (LV) vector, which was pseudotyped with a VSV-G envelop and encoded the discosoma red fluorescent protein (dsRed), to investigate which viral vector transduced the lateral hypothalamus or the amygdala more efficiently. The LV-dsRed and AAV1-GFP vector were mixed and injected into the lateral hypothalamus or into the amygdala of adult rats. The titers that were injected were 1 × 108 or 1 × 109 genomic copies of AAV1-GFP and 1 × 105 transducing units of LV-dsRed.ResultsImmunostaining for GFP and dsRed showed that AAV1-GFP transduced significantly more cells than LV-dsRed in both the lateral hypothalamus and the amygdala. In addition, the number of LV particles that were injected can not easily be increased, while the number of AAV1 particles can be increased easily with a factor 100 to 1000. Both viral vectors appear to predominantly transduce neurons.ConclusionsThis study showed that AAV1 vectors are better tools to overexpress or knockdown genes in the lateral hypothalamus and amygdala of adult rats, since more cells can be transduced with AAV1 than with LV vectors and the titer of AAV1 vectors can easily be increased to transduce the area of interest.

【 授权许可】

Unknown   
© de Backer et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.