Application of a nanotechnology antimicrobial spray to prevent lower urinary tract infection: a multicenter urology trial

Journal of Translational Medicine

Proceedings

Liming Ma¹  Hua Zhang¹  Yueping Zhang¹  Jin Chen²  Wei He³  Zhangqun Ye³  Zhicong Li⁴  Qing Yang⁵  Weiping Su⁶  Zhengzheng Ren⁷  Dongmin Wang⁸  Zhiping Wang⁸  Ping Li⁹  Xiaojun Jia^1,10  JP Chen^1,11  Wings TY Loo^1,12  Ling Qiu^1,13  Qingli Chen^1,14  Xuejing Wang^1,14  Yi Zuo^1,15  Li Lan^1,16  Siyi Li^1,17  Zhenni Ye^1,18  Xuexia Ma^1,19  Caixia Gao^2,20  Peng Wan^2,21  Biyan Wen^2,22  Xia Li^2,22  Elizabeth LY Ng^2,23  Louis WC Chow^2,23  Adrian YS Yip^2,23  Mary NB Cheung^2,23  Ling Liu^2,24  Weihong Qian^2,25  Yan Tao^2,25  Xiaofeng Shi^2,25  Nianzhen Chen^2,26 

[1] Affiliated Hospital of Nantong University, Jiangsu, PRC;Daping Hospital, Third Military Medical University, Chongqing, PRC;Department of Urology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science & Technology, Hubei, PRC;Foshan Hospital of Traditional Chinese Medicine, Guangdong, PRC;General Hospital of Guangzhou Military Command of PLA, Guangzhou, PRC;Guangzhou First Municipal People's Hospital, Guangzhou, PRC;Huai'an First Hospital Affiliated to Nanjing Medical University, Jiangsu, PRC;Institute of Urology, the Second Hospital of Lanzhou University, Lanzhou, PRC;Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, PRC;Peking University People's Hospital, Beijing, PRC;School of Chinese Medicine, The University of Hong Kong, Hong Kong SAR;School of Chinese Medicine, The University of Hong Kong, Hong Kong SAR;UNIMED Medical Institute, Hong Kong SAR;The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, PRC;The First Affiliated Hospital of Nanjing Medical University, Jiangsu, PRC;The First Affiliated Hospital of Southern Medical University (Nanfang Hospital), Guangzhou, PRC;The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PRC;The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, PRC;The Second Affiliated Hospital of Kunming Medical College, Yunnan, PRC;The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, PRC;The Second Hospital of Xi'an Jiaotong University, Xi’an, PRC;The Second Military Medical University, Changhai Hospital, Shanghai, PRC;The Third Affiliated Hospital, Sun Yat-sen University, Guangdong, PRC;UNIMED Medical Institute, Hong Kong SAR;West China Hospital, Sichuan University, Sichuan, PRC;Wuhan General Hospital of Guangzhou Military Region, Guangzhou, PRC;Xiangya Hospital of Central-south University, Changsha, PRC;

关键词: Therapy Group;    Bacteriuria;    Nitrofurazone;    Sample Fragment;    Urinary Tract Infection Patient;   

DOI  :  10.1186/1479-5876-10-S1-S14

来源: Springer

【 摘 要 】

BackgroundCatheter-associated urinary tract infection (CAUTI) is a common nosocomial device-associated infection. It is now recognized that the high infection rates were caused by the formation of biofilm on the surface of the catheters that decreases the susceptibility to antibiotics and results in anti-microbial resistance.In this study, we performed an in vitro test to explore the mechanism of biofilm formation and subsequently conducted a multi-center clinical trial to investigate the efficacy of CAUTI prevention with the application of JUC, a nanotechnology antimicrobial spray.MethodsSiliconized latex urinary catheters were cut into fragments and sterilized by autoclaving. The sterilized sample fragments were randomly divided into the therapy and control group, whereby they were sprayed with JUC and distilled water respectively and dried before use.The experimental standard strains of Escherichia coli (E. coli) were isolated from the urine samples of patients. At 16 hours and 7 days of incubation, the samples were extracted for confocal laser scanning microscopy.A total of 1,150 patients were accrued in the clinical study. Patients were randomized according to the order of surgical treatment. The odd array of patients was assigned as the therapy group (JUC), and the even array of patients was assigned as the control group (normal saline).ResultsAfter 16 hours of culture, bacterial biofilm formed on the surface of sample fragments from the control group. In the therapy group, no bacterial biofilm formation was observed on the sample fragments. No significant increase in bacterial colony count was observed in the therapy group after 7 days of incubation.On the 7th day of catheterization, urine samples were collected for bacterial culture before extubation. Significant difference was observed in the incidence of bacteriuria between the therapy group and control group (4.52% vs. 13.04%, p < 0.001).ConclusionsIn this study, the effectiveness of JUC in preventing CAUTI in a hospital setting was demonstrated in both in vitro and clinical studies.

【 授权许可】

Unknown   
© He et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.