Effect of acute administration of Pistacia lentiscus L. essential oil on rat cerebral cortex following transient bilateral common carotid artery occlusion

Lipids in Health and Disease

Research

Marianna Boi¹  Giuliano Pillolla¹  Marina Del Fiacco¹  Laura Poddighe¹  Tiziana Melis¹  Marina Quartu¹  Maria P Serra¹  Maria Collu¹  Elisabetta Murru²  Lina Cordeddu²  Sebastiano Banni²  Gianfranca Carta²  Antonio Piras²  Danilo Falconieri³ 

[1] Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, Italy;Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, Italy;Nutrisearch srl, Pula, Italy;Department of Chemical Sciences, University of Cagliari, Cittadella Universitaria, Monserrato, Italy;

关键词: Bilateral common carotid artery occlusion;    reperfusion;    DHA;    COX-2;    PEA;    OEA;    Pistacia lentiscus;    cerebral cortex;    Wistar rat;   

DOI  :  10.1186/1476-511X-11-8

 received in 2011-11-24, accepted in 2012-01-12,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundIschemia/reperfusion leads to inflammation and oxidative stress which damages membrane highly polyunsaturated fatty acids (HPUFAs) and eventually induces neuronal death. This study evaluates the effect of the administration of Pistacia lentiscus L. essential oil (E.O.), a mixture of terpenes and sesquiterpenes, on modifications of fatty acid profile and endocannabinoid (eCB) congener concentrations induced by transient bilateral common carotid artery occlusion (BCCAO) in the rat frontal cortex and plasma.MethodsAdult Wistar rats underwent BCCAO for 20 min followed by 30 min reperfusion (BCCAO/R). 6 hours before surgery, rats, randomly assigned to four groups, were gavaged either with E.O. (200 mg/0.45 ml of sunflower oil as vehicle) or with the vehicle alone.ResultsBCCAO/R triggered in frontal cortex a decrease of docosahexaenoic acid (DHA), the membrane highly polyunsaturated fatty acid most susceptible to oxidation. Pre-treatment with E.O. prevented this change and led further to decreased levels of the enzyme cyclooxygenase-2 (COX-2), as assessed by Western Blot. In plasma, only after BCCAO/R, E.O. administration increased both the ratio of DHA-to-its precursor, eicosapentaenoic acid (EPA), and levels of palmytoylethanolamide (PEA) and oleoylethanolamide (OEA).ConclusionsAcute treatment with E.O. before BCCAO/R elicits changes both in the frontal cortex, where the BCCAO/R-induced decrease of DHA is apparently prevented and COX-2 expression decreases, and in plasma, where PEA and OEA levels and DHA biosynthesis increase. It is suggested that the increase of PEA and OEA plasma levels may induce DHA biosynthesis via peroxisome proliferator-activated receptor (PPAR) alpha activation, protecting brain tissue from ischemia/reperfusion injury.

【 授权许可】

CC BY   
© Quartu et al; licensee BioMed Central Ltd. 2012