Carbon source dependent promoters in yeasts

Microbial Cell Factories

Review

Margit Winkler¹  Anton Glieder¹  Katrin Weinhandl¹  Andrea Camattari² 

[1] Austrian Centre of Industrial Biotechnology, Graz, Austria;Institute of Molecular Biotechnology, Technical University Graz, Graz, Austria;

关键词: Lactis;    Pichia Pastoris;    Recombinant Protein Production;    Glucose Repression;    Hexose Transporter;   

DOI  :  10.1186/1475-2859-13-5

 received in 2013-10-01, accepted in 2013-12-16,  发布年份 2014

来源: Springer

【 摘 要 】

Budding yeasts are important expression hosts for the production of recombinant proteins.The choice of the right promoter is a crucial point for efficient gene expression, as most regulations take place at the transcriptional level. A wide and constantly increasing range of inducible, derepressed and constitutive promoters have been applied for gene expression in yeasts in the past; their different behaviours were a reflection of the different needs of individual processes.Within this review we summarize the majority of the large available set of carbon source dependent promoters for protein expression in yeasts, either induced or derepressed by the particular carbon source provided. We examined the most common derepressed promoters for Saccharomyces cerevisiae and other yeasts, and described carbon source inducible promoters and promoters induced by non-sugar carbon sources. A special focus is given to promoters that are activated as soon as glucose is depleted, since such promoters can be very effective and offer an uncomplicated and scalable cultivation procedure.

【 授权许可】

Unknown   
© Weinhandl et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.