Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis

Microbial Cell Factories

Research

Jonathan Meade¹  Michael M Domach¹  Mohammad M Ataai²  Patrick Bartlow²  Parvez Akhtar³  Saleem A Khan³  Ram Narayan Trivedi³ 

[1] Department Chemical Engineering, Carnegie Mellon University, 15213, Pittsburgh, PA, USA;Department Chemical Engineering, University of Pittsburgh, 15219, Pittsburgh, PA, USA;Microbiology & Molecular Genetics, University of Pittsburgh School of Medicine, 15219, Pittsburgh, PA, USA;

关键词: Pyruvate Kinase;    Malic Enzyme;    Transaldolase;    Phosphoglycerate Mutase;    Hexose Monophosphate;   

DOI  :  10.1186/s12934-015-0212-x

 received in 2014-11-04, accepted in 2015-02-19,  发布年份 2015

来源: Springer

【 摘 要 】

When the replication of a plasmid based on sucrose selection is deregulated via the inc1 and inc2 mutations, high copy numbers (7,000 or greater) are attained while the growth rate on minimal medium is negligibly affected. Adaptions were assumed to be required in order to sustain the growth rate. Proteomics indicated that indeed a number of adaptations occurred that included increased expression of ribosomal proteins and 2-oxoglutarate dehydrogenase. The operating space prescribed by a basic flux model that maintained phenotypic traits (e.g. growth, byproducts, etc.) within typical bounds of resolution was consistent with the flux implications of the proteomic changes.

【 授权许可】

Unknown   
© Meade et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.