Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia

Malaria Journal

Case Report

Jonathan Wee Kent Liew¹  Rohela Mahmud¹  Yee Ling Lau¹  Lian Huat Tan² 

[1] Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia;Sunway Medical Centre, Bandar Sunway, 46150, Petaling Jaya, Selangor, Malaysia;

关键词: Plasmodium ovale wallikeri;    PCR;    Diagnosis;    Malaysia;    Southeast Asia;   

DOI  :  10.1186/s12936-015-1070-z

 received in 2015-10-15, accepted in 2015-12-23,  发布年份 2016

来源: Springer

【 摘 要 】

Plasmodium ovale is rare and not exactly known to be autochthonous in Malaysia. There are two distinct forms of the parasite, namely P. ovale curtisi (classic form) and P. ovale wallikeri (variant form). Here, the first sequence confirmed case of an imported P. ovale wallikeri infection in Malaysia is presented. Microscopy found Plasmodium parasites with morphology similar to P. ovale or Plasmodium vivax in the blood films. Further confirmation using polymerase chain reaction (PCR) targeting the small-subunit rRNA gene of the parasite was unsuccessful. Genus-specific PCR was then performed and the product was sequenced and analysed. Sequence analyses confirmed the aetiological agent as P. ovale wallikeri. New species-specific primers (rOVA1v and rOVA2v) were employed and P. ovale wallikeri was finally confirmed. The findings highlight the need to look out for imported malaria infections in Malaysia and the importance of a constantly updated and validated diagnostic technique.

【 授权许可】

CC BY   
© Liew at al. 2016