Genetic polymorphisms in TNFgenes and tuberculosis in North Indians

BMC Infectious Diseases

Research Article

Jaishriram Rathored¹  Surendra K Sharma¹  Balaram Ghosh²  Shilpy Sharma³ 

[1] Department of Medicine, All India Institute of Medical Sciences, 110029, New Delhi, India;Molecular Immunogenetics Laboratory, Institute of Genomics and Integrative Biology, Mall Road, 110007, Delhi, India;Molecular Immunogenetics Laboratory, Institute of Genomics and Integrative Biology, Mall Road, 110007, Delhi, India;University of Michigan Medical School, Biomedical Science Research Building, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA;

关键词: Tuberculosis;    Tuberculosis Patient;    Tuberculin Skin Test;    Pulmonary Tuberculosis;    Fine Needle Aspiration Cytology;   

DOI  :  10.1186/1471-2334-10-165

 received in 2010-02-07, accepted in 2010-06-10,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPulmonary tuberculosis, the most common clinical form of mycobacterial diseases, is a granulomatous disease of the lungs caused by Mycobaterium tuberculosis. A number of genes have been identified in studies of diverse origins to be important in tuberculosis. Of these, both tumor necrosis factor α (TNF-α) and lymphotoxin α (LT-α) play important immunoregulatory roles.MethodsTo investigate the association of TNF polymorphisms with tuberculosis in the Asian Indians, we genotyped five potentially functional promoter polymorphisms in the TNFA gene and a LTA_NcoI polymorphism (+252 position) of the LTA gene in a clinically well-defined cohort of North-Indian patients with tuberculosis (N = 185) and their regional controls (N = 155). Serum TNF-α (sTNF-α) levels were measured and correlated with genotypes and haplotypes.ResultsThe comparison of the allele frequencies for the various loci investigated revealed no significant differences between the tuberculosis patients and controls. Also, when the patients were sub-grouped into minimal, moderately advanced and far advanced disease on the basis of chest radiographs, TST and the presence/absence of cavitary lesions, none of the polymorphisms showed a significant association with any of the patient sub-groups. Although a significant difference was observed in the serum TNF-α levels in the patients and the controls, none of the investigated polymorphisms were found to affect the sTNF-α levels. Interestingly, it was observed that patients with minimal severity were associated with lower log sTNF-α levels when compared to the patients with moderately advanced and far advanced severity. However, none of these differences were found to be statistically significant. Furthermore, when haplotypes were analyzed, no significant difference was observed.ConclusionsThus, our findings exclude the TNF genes as major risk factor for tuberculosis in the North Indians.

【 授权许可】

CC BY   
© Sharma et al; licensee BioMed Central Ltd. 2010