Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays

BMC Genomics

Research Article

Zuowei Wu¹  Cuihua Liu¹  Baoli Zhu¹  Jing Li¹  Ruifen Zhang¹  Na Lu¹  George F Gao²  Ming Li³  Changjun Wang⁴  Hui Liao⁴  Jiaqi Tang⁴  Yongqiang Jiang⁵  Ruifu Yang⁵ 

[1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China;CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;Department of Microbiology, Third Military Medical University, Chongqing, China;Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China;

关键词: Virulent Strain;    Phylogenetic Profile;    Avirulent Strain;    Streptococcal Toxin Shock Syndrome;    Roche NimbleGen;   

DOI  :  10.1186/1471-2164-12-219

 received in 2010-12-13, accepted in 2011-05-10,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundOur previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS) is associated with specific Streptococcus suis serotype 2 (SS2) strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays.ResultsOur results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI), which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs) across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into Streptococcus pathogenicity and evolution.ConclusionsOur deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains.

【 授权许可】

Unknown   
© Wu et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.