Association of mannose-binding lectin gene polymorphisms with the development of pulmonary tuberculosis in China

BMC Infectious Diseases

Research Article

Wu-Juan Ban¹  Yan-Ling Guo¹  Guang-Li Shi¹  Yang Liu²  Qi Sun³ 

[1] Clinical laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, No. 97 Machang, Tongzhou District, 101149, Beijing, People’s Republic of China;Laboratory of Epidemiology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149, Beijing, China;Molecular biology laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, 101149, China;

关键词: Mannose-binding lectin (MBL);    Polymorphism;    Susceptibility;    Tuberculosis;   

DOI  :  10.1186/s12879-017-2310-3

 received in 2016-08-05, accepted in 2017-03-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMannose-binding lectin (MBL) is an important protein in the lectin pathway of the immune system. This study explores the association between MBL polymorphism and the susceptibility to tuberculosis (TB). The association between the MBL2 polymorphisms and serum MBL levels is also analyzed in the present study.MethodsA total of 112 inpatients with pulmonary TB and 120 healthy controls were recruited to participate in this case–control study. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism(PCR-RFLP) technology was used to genotype MBL gene (variants in −221Y/X and exon l codons 54 A/B). Serum MBL level was assayed by human MBL ELISA kit. Demographic data and exposure information were also obtained from the study participants.ResultsGenotypes YA/YA of MBL gene were more prevalent in the healthy control group than in the TB patient (P =0.038, OR, 0.57; 95% CI, 0.34-0.97) and genotypes XA/XA were less frequent in the healthy control group (P =0.007, OR, 6.42; 95% CI, 1.39-29.67). The resistant diplotype was more frequently found in the younger patients and retreatment cases with TB in MBL gene sites −221Y/X or codon 54 A/B. X/Y and A/B polymorphisms were strong determinants of serum MBL levels.ConclusionThe polymorphisms of MBL gene may be associated with susceptibility to TB and the recurrence of TB. The YA/YA may be a protected diplotype against TB.

【 授权许可】

CC BY   
© The Author(s). 2017