A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Cell Communication and Signaling

Methodology

Jose MiguelAndres Vaquero¹  Cristina Menchon¹  JuanCarlos Izpisua Belmonte²  Michael J Edel³ 

[1] Center of Regenerative Medicine in Barcelona, Dr. Aiguader 88, 08003, Barcelona, Spain;Center of Regenerative Medicine in Barcelona, Dr. Aiguader 88, 08003, Barcelona, Spain;Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Rd, 92037, La Jolla, California, USA;Center of Regenerative Medicine in Barcelona, Dr. Aiguader 88, 08003, Barcelona, Spain;Vall d' Hebron Research Institute, Xcelia and Banc de Sang i Teixits, Passeig Vall d'Hebron, 119 - 129, 08035, Barcelona, Spain;Affiliation with Victor Chang Cardiac Research Institute, Lowy Packer Building 405 Liverpool St, Darlinghurst New South Wales 2010, Australia;

关键词: Embryonic Stem Cell;    Pluripotent Stem Cell;    iPSC;    Pluripotent Cell;    Feeder Layer;   

DOI  :  10.1186/1478-811X-9-8

 received in 2010-11-19, accepted in 2011-04-11,  发布年份 2011

来源: Springer

【 摘 要 】

Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.

【 授权许可】

Unknown   
© Edel et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.