Preeclampsia induced by cadmium in rats is related to abnormal local glucocorticoid synthesis in placenta

Reproductive Biology and Endocrinology

Research

Shunqun Luo¹  Xiaojie Zhang²  Yinping Huang²  Sisi Chen²  Yi Guo²  Fan Wang³  Qiong Zhang³  Duyun Ye⁴ 

[1] Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology,, 430030,, Wuhan, Hubei,, China;Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Wenzhou Medical University,, 325000,, Wenzhou, Zhejiang,, China;Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University,, 325000,, Wenzhou, Zhejiang,, China;Department of Pathophysiology, Tonji Medical College, Huazhong University of Science and Technology,, 430030,, Wuhan, Hubei,, China;

关键词: Cadmium;    Preeclampsia;    Glucocorticoid synthesis;    Placenta;    Rat;   

DOI  :  10.1186/1477-7827-12-77

 received in 2014-05-08, accepted in 2014-08-02,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCadmium (Cd) is a major environmental pollutant that causes multiple adverse health effects in humans and animals. In this study, we investigated Cd-mediated toxic effects in rats during pregnancy and endocrine intervention in the placenta.MethodsWe exposed pregnant rats to intraperitoneal Cd (CdCl2) at various doses (0, 0.25, and 0.5 mg/kg BW/day) from days 5 to 19 of pregnancy and evaluated the maternal-placental-fetal parameters linked to preeclampsia. We measured the corticosterone level in rat serum and placental tissue by sensitive ELISA and also analyzed the expression of glucocorticoid synthesis enzymes in the placenta.ResultsKey features of preeclampsia (PE), including hypertension, proteinuria, glomerular endotheliosis, placental abnormalities and small fetal size, appeared in pregnant rats after injection with 0.5 mg/kg BW/day Cd. The placental corticosterone production and maternal and fetal plasma corticosterone levels were increased in rats treated with 0.5 mg/kg BW/day Cd (P <0.01). The expression of 21-hydroxylase (CYP21) and 11beta-hydroxylase (CYP11B1), enzymes essential for corticosteroid synthesis, were increased in Cd-exposed placenta (P <0.01). 11beta-hydroxysteroid dehydrogenase (11beta-HSD2), a dominant negative regulator of local glucocorticoid levels, was decreased in Cd-exposed placenta (P <0.01).ConclusionsOur study demonstrates for the first time that changes in placental glucocorticoid synthesis induced by Cd exposure during pregnancy could contribute to preeclamptic conditions in rats.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.