Elastomeric microparticles for acoustic mediated bioseparations

Journal of Nanobiotechnology

Methodology

Kevin Cushing¹  Leah M Johnson²  Margret Smith²  Gabriel P López³  C Wyatt Shields IV⁴  Kirill Efimenko⁵  Lu Gao⁶  Jan Genzer⁷ 

[1] Center for Biomedical Engineering, University of New Mexico, 210 University Blvd NE, 87131, Albuquerque, NM, USA;National Flow Cytometry Resource, Los Alamos National Laboratory, 87545, Los Alamos, NM, USA;Department of Biomedical Engineering, Duke University, 101 Science Drive, 3361 CIEMAS, 27708, Durham, NC, USA;Department of Biomedical Engineering, Duke University, 101 Science Drive, 3361 CIEMAS, 27708, Durham, NC, USA;Department of Mechanical Engineering and Materials Science, Duke University, Box 90300, Hudson Hall, 27708, Durham, NC, USA;NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Box 90271, 27708, Durham, NC, USA;Center for Biomedical Engineering, University of New Mexico, 210 University Blvd NE, 87131, Albuquerque, NM, USA;Department of Biomedical Engineering, Duke University, 101 Science Drive, 3361 CIEMAS, 27708, Durham, NC, USA;NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Box 90271, 27708, Durham, NC, USA;Department of Chemical and Biomolecular Engineering, North Carolina State University, Engineering Building 1, 911 Partners Way, 27695, Raleigh, NC, USA;Department of Mechanical Engineering and Materials Science, Duke University, Box 90300, Hudson Hall, 27708, Durham, NC, USA;NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Box 90271, 27708, Durham, NC, USA;NSF Research Triangle Materials Research Science and Engineering Center, Duke University, Box 90271, 27708, Durham, NC, USA;Department of Chemical and Biomolecular Engineering, North Carolina State University, Engineering Building 1, 911 Partners Way, 27695, Raleigh, NC, USA;

关键词: Cell separation;    Continuous cell sorting;    Acoustofluidics;    Particle synthesis;    Ultrasound standing wave;   

DOI  :  10.1186/1477-3155-11-22

 received in 2013-06-14, accepted in 2013-06-14,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAcoustophoresis has been utilized successfully in applications including cell trapping, focusing, and purification. One current limitation of acoustophoresis for cell sorting is the reliance on the inherent physical properties of cells (e.g., compressibility, density) instead of selecting cells based upon biologically relevant surface-presenting antigens. Introducing an acoustophoretic cell sorting approach that allows biochemical specificity may overcome this limitation, thus advancing the value of acoustophoresis approaches for both the basic research and clinical fields.ResultsThe results presented herein demonstrate the ability for negative acoustic contrast particles (NACPs) to specifically capture and transport positive acoustic contrast particles (PACPs) to the antinode of an ultrasound standing wave. Emulsification and post curing of pre-polymers, either polydimethylsiloxane (PDMS) or polyvinylmethylsiloxane (PVMS), within aqueous surfactant solution results in the formation of stable NACPs that focus onto pressure antinodes. We used either photochemical reactions with biotin-tetrafluorophenyl azide (biotin-TFPA) or end-functionalization of Pluronic F108 surfactant to biofunctionalize NACPs. These biotinylated NACPs bind specifically to streptavidin polystyrene microparticles (as cell surrogates) and transport them to the pressure antinode within an acoustofluidic chip.ConclusionTo the best of our knowledge, this is the first demonstration of using NACPs as carriers for transport of PACPs in an ultrasound standing wave. By using different silicones (i.e., PDMS, PVMS) and curing chemistries, we demonstrate versatility of silicone materials for NACPs and advance the understanding of useful approaches for preparing NACPs. This bioseparation scheme holds potential for applications requiring rapid, continuous separations such as sorting and analysis of cells and biomolecules.

【 授权许可】

Unknown   
© Johnson et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.