Bacteremia causes hippocampal apoptosis in experimental pneumococcal meningitis

BMC Infectious Diseases

Research Article

Ian Rowland¹  Christian Østergaard²  Stephen L Leib³  Christian T Brandt⁴ 

[1] Danish Research Centre for Magnetic Resonance, Copenhagen University Hospital Hvidovre, Hvidovre, Denmark;Department of Radiology, University of Wisconsin, Madison, Wisconsin, USA;Department of Clinical Microbiology, Copenhagen University Hospital Herlev, Herlev, Denmark;National Center for Antimicrobials and Infection Control, Statens Serum Institute, Copenhagen, Denmark;Institute for Infectious Diseases, University of Bern, Bern, Switzerland;National Center for Antimicrobials and Infection Control, Statens Serum Institute, Copenhagen, Denmark;Copenhagen HIV Programme, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark;

关键词: Meningitis;    Dentate Gyrus;    Bacterial Meningitis;    Pneumococcal Meningitis;    Disease Severity Score;   

DOI  :  10.1186/1471-2334-10-1

 received in 2009-08-11, accepted in 2010-01-03,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundBacteremia and systemic complications both play important roles in brain pathophysiological alterations and the outcome of pneumococcal meningitis. Their individual contributions to the development of brain damage, however, still remain to be defined.MethodsUsing an adult rat pneumococcal meningitis model, the impact of bacteremia accompanying meningitis on the development of hippocampal injury was studied. The study comprised of the three groups: I. Meningitis (n = 11), II. meningitis with attenuated bacteremia resulting from iv injection of serotype-specific pneumococcal antibodies (n = 14), and III. uninfected controls (n = 6).ResultsPneumococcal meningitis resulted in a significantly higher apoptosis score 0.22 (0.18-0.35) compared to uninfected controls (0.02 (0.00-0.02), Mann Whitney test, P = 0.0003). Also, meningitis with an attenuation of bacteremia by antibody treatment resulted in significantly reduced apoptosis (0.08 (0.02-0.20), P = 0.01) as compared to meningitis.ConclusionsOur results demonstrate that bacteremia accompanying meningitis plays an important role in the development of hippocampal injury in pneumococcal meningitis.

【 授权许可】

CC BY   
© Østergaard et al; licensee BioMed Central Ltd. 2010