Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells

BMC Cell Biology

Research Article

Hirohisa Shiraishi¹  Ayako Ohashi-Kobayashi¹  Takahiro Tanji¹  Yusuke Kobayashi¹  Syoko Haga¹  Kenji Nishikori¹  Mai Takaya¹  Yuki Kanno¹  Shohei Mitani²  Keiko Gengyo-Ando³ 

[1]Department of Immunobiology, School of Pharmacy, Iwate Medical University, 2-1-1 Nishi-tokuta, Yahaba, 028-3694, Shiwa-gun, Iwate, Japan

[2]Department of Physiology, School of Medicine, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, 162-8666, Tokyo, Japan

[3]Department of Physiology, School of Medicine, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, 162-8666, Tokyo, Japan

[4]Present address: Saitama University Brain Science Institute, 255 Shimo-okubo, Sakura-ku, 338-8570, Saitama, Japan

关键词: ABC transporter;    Caenorhabditis elegans;    Intestine;    Lipid droplet;    Lysosome;    Yolk protein;    Endosome;    Rab7;   

DOI  :  10.1186/s12860-015-0076-2

 received in 2015-08-05, accepted in 2015-12-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe intestinal cells of Caenorhabditis elegans are filled with heterogeneous granular organelles that are associated with specific organ functions. The best studied of these organelles are lipid droplets and acidified gut granules associated with GLO-1, a homolog of the small GTPase Rab38. In this study, we characterized a subset of the intestinal granules in which HAF-4 and HAF-9 localize on the membrane. HAF-4 and HAF-9 are ATP-binding cassette (ABC) transporter proteins that are homologous to the mammalian lysosomal peptide transporter TAPL (transporter associated with antigen processing-like, ABCB9).ResultsUsing transgenic worms expressing fluorescent protein-tagged marker proteins, we demonstrated that the HAF-4- and HAF-9-localizing organelles are not lipid droplets and do not participate in yolk protein transport. They were also ruled out as GLO-1-positive acidified gut granules. Furthermore, we clarified that the late endosomal protein RAB-7 localizes to the HAF-4- and HAF-9-localizing organelles and is required for their biogenesis.ConclusionsOur results indicate that the HAF-4- and HAF-9-localizing organelles are distinct intestinal organelles associated with the endocytic pathway.

【 授权许可】

CC BY   
© Tanji et al. 2015