Metabolic engineering of Bacillus subtilis for growth on overflow metabolites

Microbial Cell Factories

Research

Armin Ehrenreich¹  Dirk Albrecht²  Hanna Meyer³  Michael Lalk³  Johannes Kabisch⁴  Thomas Schweder⁴  Isabel Pratzka⁴ 

[1] Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354, Freising, Germany;Institute for Microbiology, Ernst-Moritz-Arndt-Universität, Friedrich-Ludwig-Jahn-Str. 15, D-17487, Greifswald, Germany;Institute of Biochemistry, Ernst-Moritz-Arndt-Universität, Felix-Hausdorff-Str. 4, D-17487, Greifswald, Germany;Pharmaceutical Biotechnology, Institute of Pharmacy, Ernst-Moritz-Arndt-Universität, Felix-Hausdorff-Str. 3, D-17487, Greifswald, Germany;

关键词: Bacillus subtilis;    Metabolic engineering;    Glyoxylate cycle;    Expression system;    Fed-batch;    Acetate;    Glycolic acid;   

DOI  :  10.1186/1475-2859-12-72

 received in 2013-02-07, accepted in 2013-07-08,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe genome of the important industrial host Bacillus subtilis does not encode the glyoxylate shunt, which is necessary to utilize overflow metabolites, like acetate or acetoin, as carbon source. In this study, the operon encoding the isocitrate lyase (aceB) and malate synthase (aceA) from Bacillus licheniformis was transferred into the chromosome of B. subtilis. The resulting strain was examined in respect to growth characteristics and qualities as an expression host.ResultsOur results show that the modified B. subtilis strain is able to grow on the C2 compound acetate. A combined transcript, protein and metabolite analysis indicated a functional expression of the native glyoxylate shunt of B. lichenifomis in B. subtilis. This metabolically engineered strain revealed better growth behavior and an improved activity of an acetoin-controlled expression system.ConclusionsThe glyoxylate shunt of B. licheniformis can be functionally transferred to B. subtilis. This novel strain offers improved properties for industrial applications, such as growth on additional carbon sources and a greater robustness towards excess glucose feeding.

【 授权许可】

Unknown   
© Kabisch et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.