Mucosal vaccine delivery by non-recombinant spores of Bacillus subtilis

Microbial Cell Factories

Review

Maurilio De Felice¹  Ezio Ricca¹  Loredana Baccigalupi¹  Giuseppina Cangiano¹  Rachele Isticato¹ 

[1] Department of Biology, Federico II University of Naples, 80126, via Cinthia, Naples, Italy;

关键词: Vaccine vehicles;    Vaccine delivery;    Drug delivery;    Surface display;   

DOI  :  10.1186/s12934-014-0115-2

 received in 2014-06-17, accepted in 2014-07-31,  发布年份 2014

来源: Springer

【 摘 要 】

Development of mucosal vaccines strongly relies on an efficient delivery system and, over the years, a variety of approaches based on phages, bacteria or synthetic nanoparticles have been proposed to display and deliver antigens. The spore of Bacillus subtilis displaying heterologous antigens has also been considered as a mucosal vaccine vehicle, and shown able to conjugate some advantages of live microrganisms with some of synthetic nanoparticles. Here we review the use of non-recombinant spores of B. subtilis as a delivery system for mucosal immunizations. The non-recombinant display is based on the adsorption of heterologous molecules on the spore surface without the need of genetic manipulations, thus avoiding all concerns about the use and environmental release of genetically modified microorganisms. In addition, adsorbed molecules are stabilized and protected by the interaction with the spore, suggesting that this system could reduce the rapid degradation of the antigen, often observed with other delivery systems and identified as a major drawback of mucosal vaccines.

【 授权许可】

CC BY   
© Ricca et al.; licensee BioMed Central Ltd. 2014