Assessment of hematopoietic failure due to Rpl11 deficiency in a zebrafish model of Diamond-Blackfan anemia by deep sequencing

BMC Genomics

Research Article

Qian Zhang¹  Zhaojun Zhang¹  Xiangdong Fang¹  Wanguang Zhang²  Yang Zhou³  Haibo Jia³  Qiong Jia³  Tao Cheng⁴  Yang Wan⁴  Weiping Yuan⁴  Xiaofan Zhu⁴ 

[1] CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 100101, Beijing, China;Hepatic Surgery Center Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, China;Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Center for Human Genome Research, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, China;State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 300020, Tianjin, China;

关键词: Zebrafish;    Hematopoiesis;    Rpl11;    RNA-Seq;    Transcriptome;    DBA;   

DOI  :  10.1186/1471-2164-14-896

 received in 2013-05-20, accepted in 2013-12-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundDiamond–Blackfan anemia is a rare congenital red blood cell dysplasia that develops soon after birth. RPL11 mutations account for approximately 4.8% of human DBA cases with defective hematopoietic phenotypes. However, the mechanisms by which RPL11 regulates hematopoiesis in DBA remain elusive. In this study, we analyzed the transcriptome using deep sequencing data from an Rpl11-deficient zebrafish model to identify Rpl11-mediated hematopoietic failure and investigate the underlying mechanisms.ResultsWe characterized hematological defects in Rpl11-deficient zebrafish embryos by identifying affected hematological genes, hematopoiesis-associated pathways, and regulatory networks. We found that hemoglobin biosynthetic and hematological defects in Rpl11-deficient zebrafish were related to dysregulation of iron metabolism-related genes, including tfa, tfr1b, alas2 and slc25a37, which are involved in heme and hemoglobin biosynthesis. In addition, we found reduced expression of the hematopoietic stem cells (HSC) marker cmyb and HSC transcription factors tal1 and hoxb4a in Rpl11-deficient zebrafish embryos, indicating that the hematopoietic defects may be related to impaired HSC formation, differentiation, and proliferation. However, Rpl11 deficiency did not affect the development of other blood cell lineages such as granulocytes and myelocytes.ConclusionWe identified hematopoietic failure of Rpl11-deficient zebrafish embryos using transcriptome deep sequencing and elucidated potential underlying mechanisms. The present analyses demonstrate that Rpl11-deficient zebrafish may serve as a model of DBA and may provide insights into the pathogenesis of mutant RPL11-mediated human DBA disease.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.