A proteogenomic update to Yersinia: enhancing genome annotation

BMC Genomics

Research Article

Samuel H Payne¹  Rembert Pieper¹  Shih-Ting Huang¹ 

[1] J Craig Venter Institute, 9704 Medical Center Drive, 20850, Rockville, MD, USA;

关键词: Signal Peptide;    Genome Annotation;    Yersinia Pestis;    Hydrophobic Patch;    Signal Peptide Cleavage;   

DOI  :  10.1186/1471-2164-11-460

 received in 2010-01-29, accepted in 2010-08-05,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundModern biomedical research depends on a complete and accurate proteome. With the widespread adoption of new sequencing technologies, genome sequences are generated at a near exponential rate, diminishing the time and effort that can be invested in genome annotation. The resulting gene set contains numerous errors in even the most basic form of annotation: the primary structure of the proteins.ResultsThe application of experimental proteomics data to genome annotation, called proteogenomics, can quickly and efficiently discover misannotations, yielding a more accurate and complete genome annotation. We present a comprehensive proteogenomic analysis of the plague bacterium, Yersinia pestis KIM. We discover non-annotated genes, correct protein boundaries, remove spuriously annotated ORFs, and make major advances towards accurate identification of signal peptides. Finally, we apply our data to 21 other Yersinia genomes, correcting and enhancing their annotations.ConclusionsIn total, 141 gene models were altered and have been updated in RefSeq and Genbank, which can be accessed seamlessly through any NCBI tool (e.g. blast) or downloaded directly. Along with the improved gene models we discover new, more accurate means of identifying signal peptides in proteomics data.

【 授权许可】

CC BY   
© Payne et al; licensee BioMed Central Ltd. 2010