Progeny-testing of full-sibs IBD in a SSC2 QTL region highlights epistatic interactions for fatness traits in pigs

BMC Genetics

Research Article

Yvon Billon¹  Marie-Pierre Sanchez²  Jean-Pierre Bidanel²  Juliette Riquet³  Nathalie Iannuccelli³  Denis Milan³  Katia Fève³  Hélène Gilbert⁴  Flavie Tortereau⁵ 

[1] INRA, UE967 Génétique Expérimentale en Productions Animales, 17700, Surgères, France;INRA, UMR1313 Génétique Animale et Biologie Intégrative, 78350, Jouy-en-Josas, France;INRA, UMR444 Laboratoire de Génétique Cellulaire, 31326, Castanet-Tolosan, France;INRA, UMR444 Laboratoire de Génétique Cellulaire, 31326, Castanet-Tolosan, France;INRA, UMR1313 Génétique Animale et Biologie Intégrative, 78350, Jouy-en-Josas, France;INRA, UMR444 Laboratoire de Génétique Cellulaire, 31326, Castanet-Tolosan, France;Wageningen University, Animal Breeding and Genetics Group, 6700AH, Wageningen, The Netherlands;

关键词: Wild Boar;    Epistatic Interaction;    Backfat Thickness;    Large White;    Fatness Trait;   

DOI  :  10.1186/1471-2156-12-92

 received in 2011-08-18, accepted in 2011-10-27,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMany QTL have been detected in pigs, but very few of them have been fine-mapped up to the causal mutation. On SSC2, the IGF2-intron3-G3072A mutation has been described as the causative polymorphism for a QTL underlying muscle mass and backfat deposition, but further studies have demonstrated that at least one additional QTL should segregate downstream of this mutation. A marker-assisted backcrossing design was set up in order to confirm the segregation of this second locus, reduce its confidence interval and better understand its mode of segregation.ResultsFive recombinant full-sibs, with genotype G/G at the IGF2 mutation, were progeny-tested. Only two of them displayed significant QTL for fatness traits although four inherited the same paternal and maternal chromosomes, thus exhibiting the same haplotypic contrast in the QTL region. The hypothesis of an interaction with another region in the genome was proposed to explain these discrepancies and after a genome scan, four different regions were retained as potential interacting regions with the SSC2 QTL. A candidate interacting region on SSC13 was confirmed by the analysis of an F2 pedigree, and in the backcross pedigree one haplotype in this region was found to mask the SSC2 QTL effect.ConclusionsAssuming the hypothesis of interactions with other chromosomal regions, the QTL could be unambiguously mapped to a 30 cM region delimited by recombination points. The marker-assisted backcrossing design was successfully used to confirm the segregation of a QTL on SSC2 and, because full-sibs that inherited the same alleles from their two parents were analysed, the detection of epistatic interactions could be performed between alleles and not between breeds as usually done with the traditional Line-Cross model. Additional analyses of other recombinant sires should provide more information to further improve the fine-mapping of this locus, and confirm or deny the interaction identified between chromosomes 2 and 13.

【 授权许可】

CC BY   
© Tortereau et al; licensee BioMed Central Ltd. 2011