A distinctive 'microbial signature' in celiac pediatric patients

BMC Microbiology

Research Article

Maria Barbato¹  Salvatore Cucchiara¹  Giulia Maiella¹  Giovanni Di Nardo¹  Monica Proietti Checchi²  Catia Longhi²  Valerio Iebba²  Maria Pia Conte²  Valentina Totino²  Serena Schippa² 

[1] Department of Pediatrics, "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy;Department of Public Health Sciences, 'Sapienza' University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy;

关键词: Celiac Disease;    Celiac Disease Patient;    Polymerase Chain Reaction Amplicon;    Celiac Patient;    Touchdown Polymerase Chain Reaction;   

DOI  :  10.1186/1471-2180-10-175

 received in 2010-03-04, accepted in 2010-06-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCeliac Disease (CD) is an autoimmune disorder of the small intestine in which dietary gluten ingestion leads to a chronic enteropathy. Recently, scientific evidence suggested a potential role of gut microbiota in CD. To have a snapshot of dominant duodenal microbiota we analyzed the mucosa-associated microbiota of 20 children with CD, before and after a gluten-free diet (GFD) regimen, and of 10 controls. Total DNA was extracted from duodenal biopsies and amplification products of 16S ribosomal DNA were compared by temporal temperature gradient gel electrophoresis (TTGE). TTGE profiles were analyzed by statistical multivariate analysis.ResultsThe average number of bands in TTGE profiles was significantly higher (P < 0.0001) in active (n.b. 16.7 ± 0.7) and inactive states (n.b. 13.2 ± 0.8) than in controls (n.b. 3.7 ± 1.3). Mean interindividual similarity index was 54.9% ± 14.9% for active disease, 55.6% ± 15.7% for remission state and 21.8% ± 30.16% for controls. Similarity index between celiac children before and after GFD treatment was 63.9% ± 15.8%. Differences in microbiota biodiversity were among active and remission state (P = 0.000224) and amid active CD and controls (P < 0.001). Bacteroides vulgatus and Escherichia coli were detected more often in CD patients than in controls (P < 0.0001).ConclusionsOverall, the results highlighted a peculiar microbial TTGE profile and a significant higher biodiversity in CD pediatric patients' duodenal mucosa. The possible pathophysiological role of these microbial differences needs further characterization.

【 授权许可】

CC BY   
© Schippa et al; licensee BioMed Central Ltd. 2010