A non-invasive tool for detecting cervical cancer odor by trained scent dogs

BMC Cancer

Research Article

Teresita Rojas-Sánchez¹  Raúl García-Palacios²  Irma Hernández-Gallardo³  Héctor Guerrero-Flores³  Jesús Badillo-Romero⁴  Cindy Bandala⁵  Juan Rosales-Guevara⁶  Víctor Huerta-Padilla⁷  Daniel Marrero-Rodríguez⁷  Víctor Arana-Conejo⁷  Mónica Mendoza-Rodríguez⁷  Ricardo López-Romero⁷  Teresa Apresa-García⁷  Miriam Rodríguez-Esquivel⁷  Pablo Romero-Morelos⁷  Verónica Sánchez-Albor⁷  Osvaldo Mata⁷  Mauricio Salcedo⁷  Andrea Martínez-Castillo⁷  Keiko Taniguchi⁷  Alejandro Sánchez-Pérez⁸  David Flores-Villegas⁸  Ónix Garay-Villar⁹  Artfy Bandera-Calderón^1,10 

[1] Centro Colposcópico de Docencia e Investigación, A.C., Mexico, City, Mexico;Clínica de la Mujer y Medicina Perinatal, Col. Roma Norte, Mexico City, Mexico;Coordinación de Prevención y Atención a la Salud, Delegación Sur (Instituto Mexicano del Seguro Social) IMSS, Mexico City, Mexico;Departamento de Anatomía Patológica, Hospital General de Zona Troncoso, Mexico City, Mexico;División de Neurociencias, Instituto Nacional de Rehabilitación (INR), Secretaría de Salud (S.S.), Mexico City, Mexico;Facultad de Química, Universidad La Salle, Mexico City, Mexico;Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, UMAE Hospital de Oncología, CMN- SXXI-IMSS, Av. Cuauhtémoc 330, Col. Doctores, Del. Cuauhtémoc, 06720, Mexico City, Mexico;PEC de México, S.A. de C.V., Mexico City, Mexico;Servicio de Braquiterapia, UMAE Hospital de Oncología, CMN-SXXI-IMSS, Mexico City, Mexico;Servicio de Oncología, Hospital General de Zona y de Medicina Familiar No. 5, IMSS, Taxco, Guerrero, Mexico;

关键词: Cervical Cancer;    Volatile Organic Compound;    Endometrial Cancer;    Bacterial Vaginosis;    Adsorbent Material Sample;   

DOI  :  10.1186/s12885-016-2996-4

 received in 2015-07-09, accepted in 2016-12-13,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCervical Cancer (CC) has become a public health concern of alarming proportions in many developing countries such as Mexico, particularly in low income sectors and marginalized regions. As such, an early detection is a key medical factor in improving not only their population’s quality of life but also its life expectancy. Interestingly, there has been an increase in the number of reports describing successful attempts at detecting cancer cells in human tissues or fluids using trained (sniffer) dogs. The great odor detection threshold exhibited by dogs is not unheard of. However, this represented a potential opportunity to develop an affordable, accessible, and non-invasive method for detection of CC.MethodsUsing clicker training, a male beagle was trained to recognize CC odor. During training, fresh CC biopsies were used as a reference point. Other samples used included cervical smears on glass slides and medical surgical bandages used as intimate sanitary pads by CC patients. A double-blind procedure was exercised when testing the beagle’s ability to discriminate CC from control samples.ResultsThe beagle was proven able to detect CC-specific volatile organic compounds (VOC) contained in both fresh cervical smear samples and adsorbent material samples. Beagle’s success rate at detecting and discriminating CC and non-CC odors, as indicated by specificity and sensitivity values recorded during the experiment, stood at an overall high (>90%). CC-related VOC in adsorbent materials were detectable after only eight hours of use by CC patients.ConclusionPresent data suggests different applications for VOC from the uterine cervix to be used in the detection and diagnosis of CC. Furthermore, data supports the use of trained dogs as a viable, affordable, non-invasive and, therefore, highly relevant alternative method for detection of CC lesions. Additional benefits of this method include its quick turnaround time and ease of use while remaining highly accurate and robust.

【 授权许可】

CC BY   
© The Author(s). 2017