Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris

Journal of Nanobiotechnology

Research

Luiz O Ladeira¹  Alain Couté²  Claude Yéprémian²  Humberto M Brandão³  José M Marconcini⁴  Joanne Lo⁵  Roberta Brayner⁶  Ludovic Mouton⁶  Michele M Pereira⁷  Nádia RB Raposo⁷ 

[1] Department of Physics, Federal University of Minas Gerais, 31270-901, Belo Horizonte, Brazil;Département Régulations, Développement et Diversité Moléculaire (RDDM), Muséum National d’Histoire Naturelle, 75005, Paris, France;Embrapa Dairy Cattle (CNPGL), 36038-330, Juiz de Fora, Brazil;Embrapa Instrumentation (CNPDIA), National Nanotechnology Laboratory for Agriculture (LNNA), 13560-970, São Carlos, Brazil;Institut Jacques Monod, CNRS, University Paris Diderot, 75205, Paris, France;Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), University of Paris Diderot, Sorbonne Paris Cité, 7086, Paris, France;Nucleus of Analytical Identification and Quantification (NIQUA), Federal University of Juiz de Fora, 36036-900, Juiz de Fora, Brazil;

关键词: Nanoparticle;    Uptake;    Nanotoxicity;    Microalgae;    Bioindicator;   

DOI  :  10.1186/1477-3155-12-15

 received in 2014-02-26, accepted in 2014-04-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMWCNT and CNF are interesting NPs that possess great potential for applications in various fields such as water treatment, reinforcement materials and medical devices. However, the rapid dissemination of NPs can impact the environment and in the human health. Thus, the aim of this study was to evaluate the MWCNT and cotton CNF toxicological effects on freshwater green microalgae Chlorella vulgaris.ResultsExposure to MWCNT and cotton CNF led to reductions on algal growth and cell viability. NP exposure induced reactive oxygen species (ROS) production and a decreased of intracellular ATP levels. Addition of NPs further induced ultrastructural cell damage. MWCNTs penetrate the cell membrane and individual MWCNTs are seen in the cytoplasm while no evidence of cotton CNFs was found inside the cells. Cellular uptake of MWCNT was observed in algae cells cultured in BB medium, but cells cultured in Seine river water did not internalize MWCNTs.ConclusionsUnder the conditions tested, such results confirmed that exposure to MWCNTs and to cotton CNFs affects cell viability and algal growth.

【 授权许可】

Unknown   
© Pereira et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.