Using single cell cultivation system for on-chip monitoring of the interdivision timer in Chlamydomonas reinhardtii cell cycle

Journal of Nanobiotechnology

Research

Kazunori Matsumura¹  Akihiro Hattori²  Kenji Yasuda³  Mikhail Soloviev⁴  Toshiki Yagi⁵ 

[1] Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, 153-8902, Tokyo, Japan;Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Takatsu-ku, Kawasaki, 213-0012, Kanagawa, Japan;Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Takatsu-ku, Kawasaki, 213-0012, Kanagawa, Japan;Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan;2-3-10 Kanda-Surugadai, Chiyoda, 101-0062, Tokyo, Japan;School of Biological Sciences, Royal Holloway University of London, Egham, TW20 0EX, Surrey, UK;Structural Biology, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, 606-8502, Kyoto, Japan;

关键词: Cell Volume;    Phothsynthetically Active Radiation;    Daughter Cell;    Mother Cell;    Chlamydomonas;   

DOI  :  10.1186/1477-3155-8-23

 received in 2006-11-07, accepted in 2010-09-25,  发布年份 2010

来源: Springer

【 摘 要 】

Regulation of cell cycle progression in changing environments is vital for cell survival and maintenance, and different regulation mechanisms based on cell size and cell cycle time have been proposed. To determine the mechanism of cell cycle regulation in the unicellular green algae Chlamydomonas reinhardtii, we developed an on-chip single-cell cultivation system that allows for the strict control of the extracellular environment. We divided the Chlamydomonas cell cycle into interdivision and division phases on the basis of changes in cell size and found that, regardless of the amount of photosynthetically active radiation (PAR) and the extent of illumination, the length of the interdivision phase was inversely proportional to the rate of increase of cell volume. Their product remains constant indicating the existence of an 'interdivision timer'. The length of the division phase, in contrast, remained nearly constant. Cells cultivated under light-dark-light conditions did not divide unless they had grown to twice their initial volume during the first light period. This indicates the existence of a 'commitment sizer'. The ratio of the cell volume at the beginning of the division phase to the initial cell volume determined the number of daughter cells, indicating the existence of a 'mitotic sizer'.

【 授权许可】

Unknown   
© Matsumura et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.