GLUT4 content decreases along with insulin resistance and high levels of inflammatory markers in rats with metabolic syndrome

Cardiovascular Diabetology

Original Investigation

Ubiratan F Machado¹  Maristela M Okamoto¹  Natalia M Leguisamo²  Graziela H Pinto²  Melissa M Markoski²  Alexandre M Lehnen²  Beatriz D Schaan³ 

[1] Departamento de Fisiologia e Biofísica do Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil;Laboratório de Experimentação Animal e Laboratório de Cardiologia Molecular e Celular, Instituto de Cardiologia/Fundação, Universitária de Cardiologia, Av. Princesa Isabel, 395. Bairro Santana, 90620 001, Porto Alegre, RS, Brazil;Laboratório de Experimentação Animal e Laboratório de Cardiologia Molecular e Celular, Instituto de Cardiologia/Fundação, Universitária de Cardiologia, Av. Princesa Isabel, 395. Bairro Santana, 90620 001, Porto Alegre, RS, Brazil;Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;

关键词: Monosodium glutamate;    Spontaneously hypertensive rats;    Glucose transporter 4;   

DOI  :  10.1186/1475-2840-11-100

 received in 2012-04-11, accepted in 2012-07-11,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMetabolic syndrome is characterized by insulin resistance, which is closely related to GLUT4 content in insulin-sensitive tissues. Thus, we evaluated the GLUT4 expression, insulin resistance and inflammation, characteristics of the metabolic syndrome, in an experimental model.MethodsSpontaneously hypertensive neonate rats (18/group) were treated with monosodium glutamate (MetS) during 9 days, and compared with Wistar-Kyoto (C) and saline-treated SHR (H). Blood pressure (BP) and lipid levels, C-reactive protein (CRP), interleukin 6 (IL-6), TNF-α and adiponectin were evaluated. GLUT4 protein was analysed in the heart, white adipose tissue and gastrocnemius. Studies were performed at 3 (3-mo), 6 (6-mo) and 9 (9-mo) months of age.ResultsMetS rats were more insulin resistant (p<0.001, all ages) and had higher BP (3-mo: p<0.001, 6-mo: p = 0.001, 9-mo: p = 0.015) as compared to C. At 6 months, CRP, IL-6 and TNF-α were higher (p<0.001, all comparisons) in MetS rats vs H, but adiponectin was lower in MetS at 9 months (MetS: 32 ± 2, H: 42 ± 2, C: 45 ± 2 pg/mL; p<0.001). GLUT4 protein was reduced in MetS as compared to C rats at 3, 6 and 9-mo, respectively (Heart: 54%, 50% and 57%; Gastrocnemius: 37%, 56% and 50%; Adipose tissue: 69%, 61% and 69%).ConclusionsMSG-treated SHR presented all metabolic syndrome characteristics, as well as reduced GLUT4 content, which must play a key role in the impaired glycemic homeostasis of the metabolic syndrome.

【 授权许可】

Unknown   
© Leguisamo et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.