Evaluation of the leptin receptor in human spermatozoa

Reproductive Biology and Endocrinology

Research

Shahnaz Razavi¹  Hamid Zarkesh-Esfahani²  Kamran Ghaedi³  Leila Hatami-Baroogh⁴  Somayeh Tanhaei⁵  Farzaneh Rabiee⁵  Marziyeh Tavalaee⁶  Mohammad Hossein Nasr-Esfahani⁷  Mohamad Reza Deemeh⁸ 

[1] Department of Anatomy,, Isfahan Medical University,, Isfahan, Iran;Department of Biology,, Faculty of Sciences, University of Isfahan,, Isfahan,, Iran;Department of Biology,, Faculty of Sciences, University of Isfahan,, Isfahan,, Iran;Department of Cell and Molecular Biology,, Royan Institute for Animal Biotechnology,, ACECR,, Isfahan, Iran;Department of Biology,, Science and Culture University, Tehran,, Iran;Department of Reproduction and Development,, Royan Institute for Animal Biotechnology,, ACER, Isfahan, Iran;Department of Cell and Molecular Biology,, Royan Institute for Animal Biotechnology,, ACECR,, Isfahan, Iran;Department of Reproduction and Development,, Royan Institute for Animal Biotechnology,, ACER, Isfahan, Iran;Department of Reproduction and Development,, Royan Institute for Animal Biotechnology,, ACER, Isfahan, Iran;Department of Cell and Molecular Biology,, Royan Institute for Animal Biotechnology,, ACECR,, Isfahan, Iran;Isfahan Fertility and Infertility Center,, Isfahan, Iran;Isfahan Fertility and Infertility Center,, Isfahan, Iran;

关键词: Leydig Cell;    Seminal Plasma;    Varicocele;    Semen Sample;    Male Factor Infertility;   

DOI  :  10.1186/1477-7827-8-17

 received in 2009-10-11, accepted in 2010-02-23,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundLeptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.MethodsSemen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.Resultsleptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.ConclusionThe long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.

【 授权许可】

Unknown   
© Hatami-Baroogh et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.