Engineering Corynebacterium crenatum to produce higher alcohols for biofuel using hydrolysates of duckweed (Landoltia punctata) as feedstock

Microbial Cell Factories

Research

Hai Zhao¹  Qiuli Lu²  Haifeng Su²  Maolin Wang²  Juan Jiang²  Tian Xie²  Zhao Zhao² 

[1] Bioenergy Laboratory, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, Sichuan, PR China;Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, 610064, Chengdu, Sichuan, PR China;

关键词: Corynebacterium crenatum;    Bioengineering;    Duckweed;    Higher alcohols;   

DOI  :  10.1186/s12934-015-0199-3

 received in 2014-11-15, accepted in 2015-01-26,  发布年份 2015

来源: Springer

【 摘 要 】

Early trials have demonstrated great potential for the use of duckweed (family Lemnaceae) as the next generation of energy plants for the production of biofuels. Achieving this technological advance demands research to develop novel bioengineering microorganisms that can ferment duckweed feedstock to produce higher alcohols. In this study, we used relevant genes to transfer five metabolic pathways of isoleucine, leucine and valine from the yeast Saccharomyces cerevisiae into the bioengineered microorganism Corynebacterium crenatum. Experimental results showed that the bioengineered strain was able to produce 1026.61 mg/L of 2-methyl-1-butanol by fermenting glucose, compared to 981.79 mg/L from the acid hydrolysates of duckweed. The highest isobutanol yields achieved were 1264.63 mg/L from glucose and 1154.83 mg/L from duckweed, and the corresponding highest yields of 3-methyl-1-butanol were 748.35 and 684.79 mg/L. Our findings demonstrate the feasibility of using bioengineered C. crenatum as a platform to construct a bacterial strain that is capable of producing higher alcohols. We have also shown the promise of using duckweed as the basis for developing higher alcohols, illustrating that this group of plants represents an ideal fermentation substrate that can be considered the next generation of alternative energy feedstocks.

【 授权许可】

CC BY   
© Su et al.; licensee BioMed Central. 2015