Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2

BMC Gastroenterology

Research Article

Wenchuan Wu¹  Yefei Rong¹  Wenhui Lou¹  Dayong Jin¹  Xiaolin Ni¹  Dansong Wang¹  Jianwen Hu²  Chenrui Hou² 

[1] Pancreatic Cancer Group, Department of General Surgery, Zhongshan Hospital, Fudan University, 200032, Shanghai, China;Research Center for Proteome Analysis, Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China;

关键词: Pancreatic Cancer;    Protein Spot;    Pancreatic Cancer Patient;    Myosin Light Chain Kinase;    Serum MBL2;   

DOI  :  10.1186/1471-230X-10-68

 received in 2009-12-11, accepted in 2010-06-29,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPancreatic cancer has significant morbidity and mortality worldwide. Good prognosis relies on an early diagnosis. The purpose of this study was to develop techniques for identifying cancer biomarkers in the serum of patients with pancreatic cancer.MethodsSerum samples from five individuals with pancreatic cancer and five individuals without cancer were compared. Highly abundant serum proteins were depleted by immuno-affinity column. Differential protein analysis was performed using 2-dimensional differential in-gel electrophoresis (2D-DIGE).ResultsAmong these protein spots, we found that 16 protein spots were differently expressed between the two mixtures; 8 of these were up-regulated and 8 were down-regulated in cancer. Mass spectrometry and database searching allowed the identification of the proteins corresponding to the gel spots. Up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2, which have not previously been implicated in pancreatic cancer, were observed. In an independent series of serum samples from 16 patients with pancreatic cancer and 16 non-cancer-bearing controls, increased levels of mannose-binding lectin 2 and myosin light chain kinase 2 were confirmed by western blot.ConclusionsThese results suggest that affinity column enrichment and DIGE can be used to identify proteins differentially expressed in serum from pancreatic cancer patients. These two proteins 'mannose-binding lectin 2 and myosin light chain kinase 2' might be potential biomarkers for the diagnosis of the pancreatic cancer.

【 授权许可】

Unknown   
© Rong et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.