Functional identification in Lactobacillus reuteri of a PocR-like transcription factor regulating glycerol utilization and vitamin B12 synthesis

Microbial Cell Factories

Research

Douwe Molenaar¹  Bas Teusink¹  Filipe Santos²  James Versalovic³  Jennifer K Spinler³  Delphine MA Saulnier⁴  Willem M de Vos⁵  Jeroen Hugenholtz⁶ 

[1] Center for Integrative Bioinformatics, Vrije Universiteit Amsterdam, Boelelaan 1085, 1081, Amsterdam, HV, The Netherlands;TI Food and Nutrition, Kluyver Centre for Genomics of Industrial Fermentation, and NCSB, Nieuwe Kanaal 9A, 6709, Wageningen, PA, The Netherlands;Center for Integrative Bioinformatics, Vrije Universiteit Amsterdam, Boelelaan 1085, 1081, Amsterdam, HV, The Netherlands;TI Food and Nutrition, Kluyver Centre for Genomics of Industrial Fermentation, and NCSB, Nieuwe Kanaal 9A, 6709, Wageningen, PA, The Netherlands;Department of Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands;Department of Pathology & Immunology, 1 Baylor Plaza, Baylor College of Medicine, 77030, Houston, TX, USA;Department of Pathology, Texas Children's Hospital, 1102 Bates Avenue, 77030, Houston, TX, USA;Department of Pathology & Immunology, 1 Baylor Plaza, Baylor College of Medicine, 77030, Houston, TX, USA;Department of Pathology, Texas Children's Hospital, 1102 Bates Avenue, 77030, Houston, TX, USA;NIZO Food Research, Ede, 6710, BA, the Netherlands;Laboratory of Microbiology, Wageningen University and Research Centre, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands;TI Food and Nutrition, Kluyver Centre for Genomics of Industrial Fermentation, and NCSB, Nieuwe Kanaal 9A, 6709, Wageningen, PA, The Netherlands;Department of Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands;

关键词: Empty Plasmid;    Functional Assignment;    Chemically Define Medium;    Lactobacillus Reuteri;    Glycerol Utilization;   

DOI  :  10.1186/1475-2859-10-55

 received in 2011-02-03, accepted in 2011-07-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLactobacillus reuteri harbors the genes responsible for glycerol utilization and vitamin B12 synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this island, resides a gene (lreu_1750) that based on its genomic context has been suggested to encode the regulatory protein PocR and presumably control the expression of the neighboring loci. However, this functional assignment is not fully supported by sequence homology, and hitherto, completely lacks experimental confirmation.ResultsIn this contribution, we have overexpressed and inactivated the gene encoding the putative PocR in L. reuteri. The comparison of these strains provided metabolic and transcriptional evidence that this regulatory protein controls the expression of the operons encoding glycerol utilization and vitamin B12 synthesis.ConclusionsWe provide clear experimental evidence for assigning Lreu_1750 as PocR in Lactobacillus reuteri. Our genome-wide transcriptional analysis further identifies the loci contained in the PocR regulon. The findings reported here could be used to improve the production-yield of vitamin B12, 1,3-propanediol and reuterin, all industrially relevant compounds.

【 授权许可】

Unknown   
© Santos et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.