Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat

BMC Plant Biology

Research Article

Jianmin Song¹  Genying Li¹  Yulian Li¹  Guoqi Song¹  Xianchun Xia²  Youwei Sun²  Shengnan Zhai²  Jihu Li²  Zhonghu He³  Hongqing Ling⁴ 

[1] Crop Research Institute, Shandong Academy of Agricultural Sciences, 202 Gongye Bei Road, 250100, Jinan, Shandong, China;Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China;Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China;International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, 100081, Beijing, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China;

关键词: Carotenoid biosynthesis;    RNAi;    RNA-Seq;    TILLING;    Triticum aestivum;   

DOI  :  10.1186/s12870-016-0916-z

 received in 2016-07-08, accepted in 2016-10-06,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPhytoene synthase 1 (PSY1) is the most important regulatory enzyme in carotenoid biosynthesis, whereas its function is hardly known in common wheat. The aims of the present study were to investigate Psy1 function and genetic regulation using reverse genetics approaches.ResultsTranscript levels of Psy1 in RNAi transgenic lines were decreased by 54–76 % and yellow pigment content (YPC) was reduced by 26–35 % compared with controls, confirming the impact of Psy1 on carotenoid accumulation. A series of candidate genes involved in secondary metabolic pathways and core metabolic processes responded to Psy1 down-regulation. The aspartate rich domain (DXXXD) was important for PSY1 function, and conserved nucleotides adjacent to the domain influenced YPC by regulating gene expression, enzyme activity or alternative splicing. Compensatory responses analysis indicated that three Psy1 homoeologs may be coordinately regulated under normal conditions, but separately regulated under stress. The period 14 days post anthesis (DPA) was found to be a key regulation node during grain development.ConclusionThe findings define key aspects of flour color regulation in wheat and facilitate the genetic improvement of wheat quality targeting color/nutritional specifications required for specific end products.

【 授权许可】

CC BY   
© The Author(s). 2016