Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

Microbial Cell Factories

Research

George Wang¹  Tsan-Yu Chiu¹  Yi-Lin Tsai¹  Aymerick Eudes¹  Edward E. K. Baidoo¹  Aindrila Mukhopadhyay¹  Henrik V. Scheller¹  Maxence Mouille¹  Dominique Loqué²  Jay D. Keasling³  Veronica T. Benites⁴  Lucien Roux⁵  Joshua L. Heazlewood⁶  Samuel Deutsch⁷  David S. Robinson⁷ 

[1] Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, 94608, Emeryville, CA, USA;Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA;Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, 94608, Emeryville, CA, USA;Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA;CNRS, UMR5240, Microbiologie, Adaptation et Pathogénie, Université Claude Bernard Lyon 1, INSA de Lyon, 10 rue Raphaël Dubois, 69622, Villeurbanne, France;Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, 94608, Emeryville, CA, USA;Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA;Department of Chemical & Biomolecular Engineering and Department of Bioengineering, University of California, 94720, Berkeley, CA, USA;Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle´, 2970, Hørsholm, Denmark;Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, 94608, Emeryville, CA, USA;Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA;Graduate Program, San Francisco State University, 94132, San Francisco, CA, USA;Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, 94608, Emeryville, CA, USA;Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA;Master Program, Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland;Joint BioEnergy Institute, EmeryStation East, 5885 Hollis St., 4th Floor, 94608, Emeryville, CA, USA;Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA;School of BioSciences, The University of Melbourne, 3010, Melbourne, VIC, Australia;Joint Genome Institute, 94598, Walnut Creek, CA, USA;

关键词: Yeast;    BAHD;    Antioxidant;    Therapeutics;    Flavors and fragrances;    CAPE;   

DOI  :  10.1186/s12934-016-0593-5

 received in 2016-08-31, accepted in 2016-11-06,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBAHD acyltransferases, named after the first four biochemically characterized enzymes of the group, are plant-specific enzymes that catalyze the transfer of coenzyme A-activated donors onto various acceptor molecules. They are responsible for the synthesis in plants of a myriad of secondary metabolites, some of which are beneficial for humans either as therapeutics or as specialty chemicals such as flavors and fragrances. The production of pharmaceutical, nutraceutical and commodity chemicals using engineered microbes is an alternative, green route to energy-intensive chemical syntheses that consume petroleum-based precursors. However, identification of appropriate enzymes and validation of their functional expression in heterologous hosts is a prerequisite for the design and implementation of metabolic pathways in microbes for the synthesis of such target chemicals.ResultsFor the synthesis of valuable metabolites in the yeast Saccharomyces cerevisiae, we selected BAHD acyltransferases based on their preferred donor and acceptor substrates. In particular, BAHDs that use hydroxycinnamoyl-CoAs and/or benzoyl-CoA as donors were targeted because a large number of molecules beneficial to humans belong to this family of hydroxycinnamate and benzoate conjugates. The selected BAHD coding sequences were synthesized and cloned individually on a vector containing the Arabidopsis gene At4CL5, which encodes a promiscuous 4-coumarate:CoA ligase active on hydroxycinnamates and benzoates. The various S. cerevisiae strains obtained for co-expression of At4CL5 with the different BAHDs effectively produced a wide array of valuable hydroxycinnamate and benzoate conjugates upon addition of adequate combinations of donors and acceptor molecules. In particular, we report here for the first time the production in yeast of rosmarinic acid and its derivatives, quinate hydroxycinnamate esters such as chlorogenic acid, and glycerol hydroxycinnamate esters. Similarly, we achieved for the first time the microbial production of polyamine hydroxycinnamate amides; monolignol, malate and fatty alcohol hydroxycinnamate esters; tropane alkaloids; and benzoate/caffeate alcohol esters. In some instances, the additional expression of Flavobacterium johnsoniae tyrosine ammonia-lyase (FjTAL) allowed the synthesis of p-coumarate conjugates and eliminated the need to supplement the culture media with 4-hydroxycinnamate.ConclusionWe demonstrate in this study the effectiveness of expressing members of the plant BAHD acyltransferase family in yeast for the synthesis of numerous valuable hydroxycinnamate and benzoate conjugates.

【 授权许可】

CC BY   
© The Author(s) 2016