Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis

Lipids in Health and Disease

Research

Jumat Salimon¹  Nadia Salih¹  Bashar Mudhaffar Abdullah¹ 

[1] School of Chemical Sciences and Food Technology Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Selangor, Bangi, Malaysia;

关键词: Rubber seed oil;    Linamarin;    Toxicity;    Colorimetric method;    Rats;    Shrimps;   

DOI  :  10.1186/1476-511X-11-74

 received in 2012-02-28, accepted in 2012-05-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.ResultsFTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn’t show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSOh, RSOchl+mth and RSOeth and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).ConclusionsThis can be attributed no hazardous linamarin were found in RSO.

【 授权许可】

CC BY   
© Salimon et al.; licensee BioMed Central Ltd. 2012