Repeated remote ischemic preconditioning and isoflurane anesthesia in an experimental model of renal ischemia-reperfusion injury

BMC Anesthesiology

Research Article

Mehmet Ergun¹  Roger M. L. M. Lomme¹  Michiel C. Warlé¹  Moira H. D. Bruintjes¹  Harry van Goor¹  Theo P. Menting¹  Kimberley E. Wever² 

[1] Department of Surgery, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands;Systematic Review Centre for Laboratory animal Experimentation, SYRCLE, Radboud University Medical Center, Geert Grooteplein-zuid 10, 6525 GA, Nijmegen, The Netherlands;

关键词: Anesthetic preconditioning;    Animal experiment;    Ischemia reperfusion injury;    Kidney;    Repeated remote ischemic preconditioning;   

DOI  :  10.1186/s12871-017-0310-x

 received in 2016-07-23, accepted in 2017-01-23,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIn animal studies, remote ischemic preconditioning (RIPC) and anesthetic preconditioning are successful in reducing renal ischemia reperfusion injury (IRI), however the protective effect of RIPC may be improved by repeating the RIPC stimulus.MethodsSprague-Dawley rats underwent unilateral nephrectomy followed by 30 min of renal pedicle clamping. Animals were allocated into six groups: sham, control (IRI), RepISO (daily isoflurane anesthesia), RIPC (single dose isoflurane anesthesia and single dose RIPC), RepISO + RIPC (7-day isoflurane anesthesia and single dose RIPC) and RepISO + RepRIPC (7-day isoflurane anesthesia with 7-day RIPC). RIPC was applied by 3×5 min of cuff inflation on both thighs. Serum creatinine and urea levels were measured and histology was obtained at day two.ResultsRepISO diminished renal IRI, as reflected by a significant reduction in serum creatinine levels as compared to the control group, 170 ± 74 resp. 107 ± 29 μmol/L. The other preconditioning protocols showed similar reduction in serum creatinine levels as compared to the control group. No significant differences were observed between the different preconditioning protocols. For urea levels, only RepISO + RIPC resulted in significantly lower levels as compared to the control group, 14 ± 4 resp. 22 ± 7 mmol/L (p = 0.010). In the preconditioning groups only RepISO showed less histological damage as compared to controls 1.73 ± 1.19 resp. 2.91 ± 1.22 (p = 0.032).ConclusionsIn this study no additional protective effect of repeated ischemic preconditioning was observed as compared to single dose RIPC. Repeated administration of isoflurane provided stronger protection against renal IRI as compared to single dose isoflurane.

【 授权许可】

CC BY   
© The Author(s). 2017