No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

BMC Veterinary Research

Research Article

F. Granberg¹  J. P. Nielsen²  C. K. Hjulsager³  S. E. Jorsal³  N. B. Goecke³  L. E. Larsen³  S. Rasmussen³  M. Boye⁴  Ø. Angen⁵  H. Kongsted⁶  S. E. Midgley⁷  T. K. Fischer⁷  L. D. Rasmussen⁸ 

[1] Department of Biomedical Sciences and Veterinary Public Health (BVF), Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Gronnegaardsvej 15, DK-1870, Frederiksberg, Denmark;National Veterinary Institute, Technical University of Denmark, Kemitorvet, DK-2800, Lyngby, Denmark;National Veterinary Institute, Technical University of Denmark, Kemitorvet, DK-2800, Lyngby, Denmark;Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Gronnegaardsvej 15, DK-1870, Frederiksberg, Denmark;National Veterinary Institute, Technical University of Denmark, Kemitorvet, DK-2800, Lyngby, Denmark;Statens Serum Institut (SSI), Artillerivej 5, DK-2300, Copenhagen S, Denmark;Pig Research Centre, Danish Agriculture and Food Council, Vinkelvej 13, DK-8620, Kjellerup, Denmark;Statens Serum Institut (SSI), Artillerivej 5, DK-2300, Copenhagen S, Denmark;Statens Serum Institut (SSI), Artillerivej 5, DK-2300, Copenhagen S, Denmark;National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771, Kalvehave, Denmark;

关键词: Diarrhoea;    Neonatal piglets;    NNPDS;    Virus;   

DOI  :  10.1186/s12917-017-1239-5

 received in 2017-04-07, accepted in 2017-10-30,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods.ResultsA total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each.ConclusionsOur detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.

【 授权许可】

CC BY   
© The Author(s). 2017