Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar

Malaria Journal

Research

Berit Aydin-Schmidt¹  Anders Björkman¹  Elin Edlund¹  Jackie Cook²  Andreas Mårtensson³  Iveth J González⁴  David Bell⁵  Abdullah Ali⁶  Mwinyi Msellem⁶  Majda H Nassor⁶  Ali K Abass⁶ 

[1] Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden;Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden;Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, London, UK;Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden;Global Health (IHCAR), Department of Public Health Sciences, Karolinska Institutet, SE-171 77, Stockholm, Sweden;Centre for Clinical Research Sörmland, Uppsala University, Uppsala, Sweden;Foundation for Innovative New Diagnostics (FIND), Geneva, Switzerland;Global Good /Intellectual Ventures Laboratory, Bellevue, WA, USA;Zanzibar Malaria Elimination Programme, Ministry of Health, Zanzibar, Tanzania;

关键词: LAMP;    Low-density;    Malaria;    Zanzibar;    Elimination;    Asymptomatic;    Diagnostics;   

DOI  :  10.1186/s12936-015-0573-y

 received in 2014-09-01, accepted in 2015-01-18,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAsymptomatic, low parasite density malaria infections are difficult to detect with currently available point-of-care diagnostics. This study piloted a loop-mediated isothermal amplification (LAMP) kit for field-friendly, high-throughput detection of asymptomatic malaria infections during mass screening and treatment (MSAT) in Zanzibar, a malaria pre-elimination setting.MethodsScreening took place in three known hotspot areas prior to the short rains in November. Finger-prick blood was taken for screening by rapid diagnostic test (RDT) and LAMP and collected on filter paper for subsequent polymerase chain reaction (PCR) analyses. LAMP results were compared to RDT and to PCR using McNemar’s test.ResultsApproximately 1,000 people were screened. RDT detected ten infections (1.0% (95% CI 0.3-1.6)) whilst both LAMP and PCR detected 18 (1.8% (95% CI 0.9-2.6)) infections. However, PCR identified three infections that LAMP did not detect and vice versa. LAMP testing was easy to scale-up in field conditions requiring minimal training and equipment, with results ready one to three hours after screening.ConclusionsDespite lower than expected prevalence, LAMP detected a higher number of infections than the currently used diagnostic, RDT. LAMP is a field-friendly, sensitive diagnostic test that could be useful for MSAT malaria campaigns which require quick results to enable prompt treatment.

【 授权许可】

CC BY   
© Cook et al.; licensee BioMed Central. 2015