Transformation of Botrytis cinerea by direct hyphal blasting or by wound-mediated transformation of sclerotia

BMC Microbiology

Methodology Article

Amnon Lichter¹  Shahar Ish - Shalom¹  Maggie Levy²  Aviva Gafni² 

[1] Postharvest Science of Fresh Produce, The Volcani Center, ARO, Israel;The Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel;

关键词: Botrytis cinerea;    sclerotium;    Sclerotinia sclerotiorum;    transformation;    'Bim-Lab';    electroporation;   

DOI  :  10.1186/1471-2180-11-266

 received in 2011-09-21, accepted in 2011-12-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundBotrytis cinerea is a haploid necrotrophic ascomycete which is responsible for 'grey mold' disease in more than 200 plant species. Broad molecular research has been conducted on this pathogen in recent years, resulting in the sequencing of two strains, which has generated a wealth of information toward developing additional tools for molecular transcriptome, proteome and secretome investigations. Nonetheless, transformation protocols have remained a significant bottleneck for this pathogen, hindering functional analysis research in many labs.ResultsIn this study, we tested three different transformation methods for B. cinerea: electroporation, air-pressure-mediated and sclerotium-mediated transformation. We demonstrate successful transformation with three different DNA constructs using both air-pressure- and sclerotium-mediated transformation.ConclusionsThese transformation methods, which are fast, simple and reproducible, can expedite functional gene analysis of B. cinerea.

【 授权许可】

Unknown   
© Ish-Shalom et al.; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.