Time course study of the response to LPS targeting the pig immune gene networks

BMC Genomics

Research Article

Darya Bazovkina¹  Yvon Billon²  Valérie Sautron³  Pierre Mormede³  Elena Terenina³  Amélie Sevin-Pujol³  Laure Gress³  Caroline Ydier³  Laurence Liaubet³  Nathalie Villa-Vialaneix⁴  Claire Naylies⁵  Yannick Lippi⁵ 

[1] Department of Behavioral Neurogenomics, Siberian Branch of the Russian Academy of Sciences, 630090, Novosibirsk, Russia;INRA, UE 1372 GenESI, F-17700, Surgeres, France;INRA, UMR 1388 GenPhySE, Université de Toulouse, INRA, INPT, ENVT, F-31326, Castanet-Tolosan, France;MIAT, Université de Toulouse, INRA, Castanet-Tolosan, France;Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, F-31027, Toulouse, France;

关键词: Stress;    Hypothalamic-pituitary-adrenal (HPA) axis;    Cortisol;    Time-course;    Systems biology;    Microarray;    Pig;   

DOI  :  10.1186/s12864-017-4363-5

 received in 2017-03-30, accepted in 2017-12-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundStress is a generic term used to describe non-specific responses of the body to all kinds of challenges. A very large variability in the response can be observed across individuals, depending on numerous conditioning factors like genetics, early influences and life history. As a result, there is a wide range of individual vulnerability and resilience to stress, also called robustness. The importance of robustness-related traits in breeding strategies is increasing progressively towards the production of animals with a high level of production under a wide range of climatic conditions and management systems, together with a lower environmental impact and a high level of animal welfare. The present study aims at describing blood transcriptomic, hormonal, and metabolic responses of pigs to a systemic challenge using lipopolysaccharide (LPS). The objective is to analyze the individual variation of the biological responses in relation to the activity of the HPA axis measured by the levels of plasma cortisol after LPS and ACTH in 120 juvenile Large White (LW) pigs. The kinetics of the response was measured with biological variables and whole blood gene expression at 4 time points. A multilevel statistical analysis was used to take into account the longitudinal aspect of the data.ResultsCortisol level reaches its peak 4 h after LPS injection. The characteristic changes of white blood cell count to LPS were observed, with a decrease of total count, maximal at t=+4 h, and the mirror changes in the respective proportions of lymphocytes and granulocytes. The lymphocytes / granulocytes ratio was maximal at t=+1 h. An integrative statistical approach was used and provided a set of candidate genes for kinetic studies and ongoing complementary studies focused on the LPS-stimulated inflammatory response.ConclusionsThe present study demonstrates the specific biomarkers indicative of an inflammation in swine. Furthermore, these stress responses persist for prolonged periods of time and at significant expression levels, making them good candidate markers for evaluating the efficacy of anti-inflammatory drugs.

【 授权许可】

CC BY   
© The Author(s) 2017

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