The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Microbial Cell Factories

Research

Jinjin Qiao¹  Ang Ren¹  Mingwen Zhao¹  Xiaoyu Guo¹  Zhenzhen Xing¹  Jing Zhu²  Lingdan Lian³ 

[1] Key Laboratory of Agricultural Environmental Microbiology, Microbiology Department, College of Life Sciences, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, 210095, Jiangsu, Nanjing, PR China;Key Laboratory of Agricultural Environmental Microbiology, Microbiology Department, College of Life Sciences, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, 210095, Jiangsu, Nanjing, PR China;College of Life Sciences, Nanjing Agricultural University, No.1 Weigang, 210095, Nanjing, Jiangsu, PR China;Key Laboratory of Agricultural Environmental Microbiology, Microbiology Department, College of Life Sciences, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, 210095, Jiangsu, Nanjing, PR China;Department of Bioengineering, College of Food Science, South China Agricultural University, 510642, Guangzhou, PR China;

关键词: GCN4;    Amino acids;    Nitrogen;    Ganoderma lucidum;   

DOI  :  10.1186/s12934-023-02213-z

 received in 2023-06-29, accepted in 2023-09-26,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundEdible mushrooms are delicious in flavour and rich in high-quality protein and amino acids required by humans. A transcription factor, general control nonderepressible 4 (GCN4), can regulate the expression of genes involved in amino acid metabolism in yeast and mammals. A previous study revealed that GCN4 plays a pivotal role in nitrogen utilization and growth in Ganoderma lucidum. However, its regulation is nearly unknown in mushrooms.ResultsIn this study, we found that the amino acid contents reached 120.51 mg per gram of mycelia in the WT strain under 60 mM asparagine (Asn) conditions, but decreased by 62.96% under 3 mM Asn conditions. Second, silencing of gcn4 resulted in a 54.2% decrease in amino acid contents under 60 mM Asn, especially for the essential and monosodium glutamate-like flavour amino acids. However, these effects were more pronounced under 3 mM Asn. Third, silencing of gcn4 markedly inhibited the expression of amino acid biosynthesis and transport genes. In addition, GCN4 enhanced the tricarboxylic acid cycle (TCA) and glycolytic pathway and inhibited the activity of target of rapamycin complex 1 (TORC1), thus being beneficial for maintaining amino acid homeostasis.ConclusionThis study confirmed that GCN4 contributes to maintaining the amino acid contents in mushrooms under low concentrations of nitrogen. In conclusion, our study provides a research basis for GCN4 to regulate amino acid synthesis and improve the nutrient contents of edible mushrooms.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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