Differential abundance analysis of mesocarp protein from high- and low-yielding oil palms associates non-oil biosynthetic enzymes to lipid biosynthesis

Proteome Science

Research

Boon Zean Ng¹  Fook Tim Chew²  Leona Daniela Jeffery Daim³  Wan Chin Yeap³  David Ross Appleton³  Tony Eng Keong Ooi³  Fong Chin Lee³  Ainul Masni Othman³  Harikrishna Kulaveerasingam³ 

[1] Agro-Biotechnology Institute Malaysia, National Institutes of Biotechnology Malaysia, c/o MARDI Headquarters, 43400, Serdang, Selangor, Malaysia;Department of Biological Sciences, Faculty of Science, National University of Singapore, Kent Ridge Road, 117543, Singapore, Singapore;Sime Darby Technology Centre Sdn. Bhd., UPM-MTDC Technology Centre III, Lebuh Silikon, Universiti Putra Malaysia, 1st Floor, Block B, 43400, Serdang, Selangor, Malaysia;

关键词: Oil palm;    Elaeis guineensis;    Yield;    Lipid biosynthesis;    Plant proteome;   

DOI  :  10.1186/s12953-015-0085-2

 received in 2015-08-03, accepted in 2015-11-22,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe oil palm Elaeis guineensis Jacq. which produces the highest yield per unit land area of the oil crops is the most important commercial oil crop in South East Asia. The fleshy mesocarp of oil palm fruit, where oil is mostly derived from, contains up to 90 % dry weight of oil (one of the most concentrated in plant tissues). Hence, there is attention given to gain insights into the processes of oil deposition in this oil rich tissue. For that purpose, two-dimensional differential gel electrophoresis (DIGE) coupled with western assays, were used here to analyze differential protein levels in genetically-related high-and low-yielding oil palm mesocarps.ResultsFrom the DIGE comparative analysis in combination with western analysis, 41 unique differentially accumulated proteins were discovered. Functional categorization of these proteins placed them in the metabolisms of lipid, carbohydrate, amino acids, energy, structural proteins, as well as in other functions. In particular, higher abundance of fructose-1,6-biphosphate aldolase combined with reduced level of triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase may be indicative of important flux balance changes in glycolysis, while amino acid metabolism also appeared to be closely linked with oil yield.ConclusionsForty-one proteins in several important biological pathways were identified as exhibiting differential in abundance at critical oil production stages. These confirm that oil yield is a complex trait involving the regulation of genes in multiple biological pathways. The results also provide insights into key control points of lipid biosynthesis in oil palm and can assist in the development of genetic markers for use in oil palm breeding programmes.

【 授权许可】

CC BY   
© Ooi et al. 2015