Sequence variation and haplotypes of lipoxygenase gene LOX-1 in the Australian barley varieties

BMC Genetics

Research Article

Stefan Harasymow¹  Blakely Paynter¹  Chengdao Li²  Dianxing Wu³  Xiaoli Shu³  Hongxia Ye⁴  Michael Jones⁵  Xiao-Qi Zhang⁵ 

[1] Department of Agriculture and Food, Government of Western Australia, 3 Baron-Hay Court, 6151, South Perth, WA, Australia;Department of Agriculture and Food, Government of Western Australia, 3 Baron-Hay Court, 6151, South Perth, WA, Australia;The State Agricultural Biotechnology Centre, Murdoch University, 6150, Murdoch, WA, Australia;State Key Lab of Rice Biology and Key Lab of the Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, 310029, Hangzhou, P.R. Chin;State Key Lab of Rice Biology and Key Lab of the Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, 310029, Hangzhou, P.R. Chin;Department of Agriculture and Food, Government of Western Australia, 3 Baron-Hay Court, 6151, South Perth, WA, Australia;The State Agricultural Biotechnology Centre, Murdoch University, 6150, Murdoch, WA, Australia;

关键词: Lipoxygenase-1;    Structural gene;    Haplotype;    Malting quality;    Hordeum vulgare;   

DOI  :  10.1186/1471-2156-15-36

 received in 2013-12-10, accepted in 2014-03-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundLipoxygenases are a family of enzymes which catalyse the hydroperoxidation of polyunsaturated fatty acids with a cis, cis-1,4-pentadiene to form conjugated hydroperoxydienes. Lipoxygenase-1 (LOX-1) in barley worsens the flavour and foam stability of beer. It has become a major selection criteria for malting quality in the last few years.ResultsLipoxygenase activity was investigated in 41 Australian barley cultivars and advanced breeding lines released since the 1950s; the cultivars differed markedly, ranging from 22.3 to 46.5 U/g. The structural gene and its promoter of lipoxygenase-1 were sequenced from the barley varieties representing different levels of LOX. Based on the analysis of nucleotide and deduced amino acid sequences, two major haplotypes were identified. Barley varieties with lower LOX were classified into three categories based on their pedigrees and sequence variations in the structural gene: (1) barley varieties derived from Canadian varieties with the pre-harvest sprouting susceptible allele, (2) Skiff and Hindmarsh with unique haplotype in the structural gene, and (3) Gairdner and Onslow with an unknown mechanism.ConclusionLipoxygenase activity has been reduced in the malting barley cultivars in the last 60 years although it is only recognized as a malting quality trait recently. There are clear haplotypes of the lipoxygenase structual gene. The polymorphisms detected in the structural gene can be used to design molecular markers for selection of low LOX haplotype. Other mechanisms also existed for controlling lipoxygenase activity. The results suggest that it is possible to develop barley varieties with lower LOX by combination of low LOX-1 haplotype and other trans-regulation factors.

【 授权许可】

Unknown   
© Ye et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.