Identification of epidermal growth factor receptor-positive glioblastoma using lipid-encapsulated targeted superparamagnetic iron oxide nanoparticles in vitro

Journal of Nanobiotechnology

Research

Wan-Zhen Huang¹  Ray-Jade Chen²  Fei-Ting Hsu³  Ping-Huei Tsai³  Huai-Lu Chen⁴  Ahmed Atef Ahmed Ali⁴  Gilbert Aaron Lee⁴  Cheng-Yu Chen⁵  Yu-Chieh Jill Kao⁶  Chia-Feng Lu⁷  Hua-Shan Liu⁸ 

[1] Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan;Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan;Translational Imaging Research Center, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Medical Imaging, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan;Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Translational Imaging Research Center, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan;Translational Imaging Research Center, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan;Department of Medical Imaging, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan;Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Translational Imaging Research Center, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Translational Imaging Research Center, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan;Translational Imaging Research Center, College of Medicine, Taipei Medical University, Taipei, Taiwan;School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan;

关键词: Epidermal growth factor receptor (EGFR);    Targeted superparamagnetic iron oxide (SPIO) nanoparticle;    Magnetic resonance imaging (MRI);    Glioblastoma;    Lipid-encapsulated nanoparticle;   

DOI  :  10.1186/s12951-017-0313-2

 received in 2017-06-02, accepted in 2017-10-30,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTargeted superparamagnetic iron oxide (SPIO) nanoparticles have emerged as a promising biomarker detection tool for molecular magnetic resonance (MR) image diagnosis. To identify patients who could benefit from Epidermal growth factor receptor (EGFR)-targeted therapies, we introduce lipid-encapsulated SPIO nanoparticles and hypothesized that anti-EGFR antibody cetuximab conjugated of such nanoparticles can be used to identify EGFR-positive glioblastomas in non-invasive T2 MR image assays. The newly introduced lipid-coated SPIOs, which imitate biological cell surface and thus inherited innate nonfouling property, were utilized to reduce nonspecific binding to off-targeted cells and prevent agglomeration that commonly occurs in nanoparticles.ResultsThe synthesized targeted EGFR-antibody-conjugated SPIO (EGFR-SPIO) nanoparticles were characterized using dynamic light scattering, zeta potential assays, gel electrophoresis mobility shift assays, transmission electron microscopy (TEM) images, and cell line affinity assays, and the results showed that the conjugation was successful. The targeting efficiency of the synthesized EGFR-SPIO nanoparticles was confirmed through Prussian blue staining and TEM images by using glioblastoma cell lines with high or low EGFR expression levels. The EGFR-SPIO nanoparticles preferentially targeted U-251 cells, which have high EGFR expression, and were internalized by cells in a prolonged incubation condition. Moreover, the T2 MR relaxation time of EGFR-SPIO nanoparticles could be used for successfully identifying glioblastoma cells with elevated EGFR expression in vitro and distinguishing U-251 cells from U-87MG cells, which have low EFGR expression.ConclusionThese findings reveal that the lipid-encapsulated EGFR-SPIO nanoparticles can specifically target cells with elevated EGFR expression in the three tested human glioblastoma cell lines. The results of this study can be used for noninvasive molecular MR image diagnosis in the future.

【 授权许可】

CC BY   
© The Author(s) 2017