Consistent levels of A-to-I RNA editing across individuals in coding sequences and non-conserved Alu repeats

BMC Genomics

Research Article

Sivan Osenberg¹  Michal Safran¹  Nurit Paz-Yaacov¹  Ninette Amariglio¹  Gideon Rechavi¹  Aviv Barzilai²  Eli Eisenberg³  Erez Y Levanon⁴  Shoshana Greenberger⁵ 

[1] Cancer Research Center, Sheba Medical Center, Tel-Ha'Shomer, and Tel-Aviv University, Israel;Department of Dermatology, Sheba Medical Center, Tel-Hashomer, Israel;Raymond and Beverly Sackler School of Physics and Astronomy, Tel-Aviv University, 69978, Tel Aviv, Israel;The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 52900, Ramat-Gan, Israel;Vascular Biology Program and Department of Surgery, Children's Hospital Boston and Harvard Medical School, 02115, Boston, MA, USA;Department of Dermatology, Sheba Medical Center, Tel-Hashomer, Israel;

关键词: Skin Sample;    Editing Site;    Editing Event;    Short Intersperse Nuclear Element;    Editing Efficiency;   

DOI  :  10.1186/1471-2164-11-608

 received in 2010-04-28, accepted in 2010-10-28,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAdenosine to inosine (A-to-I) RNA-editing is an essential post-transcriptional mechanism that occurs in numerous sites in the human transcriptome, mainly within Alu repeats. It has been shown to have consistent levels of editing across individuals in a few targets in the human brain and altered in several human pathologies. However, the variability across human individuals of editing levels in other tissues has not been studied so far.ResultsHere, we analyzed 32 skin samples, looking at A-to-I editing level in three genes within coding sequences and in the Alu repeats of six different genes. We observed highly consistent editing levels across different individuals as well as across tissues, not only in coding targets but, surprisingly, also in the non evolutionary conserved Alu repeats.ConclusionsOur findings suggest that A-to-I RNA-editing of Alu elements is a tightly regulated process and, as such, might have been recruited in the course of primate evolution for post-transcriptional regulatory mechanisms.

【 授权许可】

Unknown   
© Greenberger et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.