Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells

Cell Communication and Signaling

Research

Christoph Gelhaus¹  Matthias Leippe¹  Ralph Lucius²  Marcus Lettau³  Melanie Nebendahl³  Hendrik Schmidt³  Ottmar Janssen³  Dietrich Kabelitz³ 

[1] Department of Zoophysiology, Zoological Institute, Christian-Albrechts-University, Kiel, Germany;Institute of Anatomy, Christian-Albrechts-University, Kiel, Germany;Institute of Immunology, Christian-Albrechts-University, UK S-H Campus Kiel, Kiel, Germany;

关键词: Natural Killer Cell;    Cytotoxic Effector;    Intact Vesicle;    Secretory Lysosome;    Natural Killer Cell Population;   

DOI  :  10.1186/1478-811X-9-4

 received in 2010-12-01, accepted in 2011-01-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCytotoxic cells of the immune system have evolved a lysosomal compartment to store and mobilize effector molecules. In T lymphocytes and NK cells, the death factor FasL is one of the characteristic marker proteins of these so-called secretory lysosomes, which combine properties of conventional lysosomes and exocytotic vesicles. Although these vesicles are crucial for immune effector function, their protein content in T cells has so far not been investigated in detail.ResultsIn the present study, intact membranous vesicles were enriched from homogenates of polyclonally activated T cells and initially characterized by Western blotting and electron microscopic inspection. The vesicular fraction that contained the marker proteins of secretory lysosomes was subsequently analyzed by 2D electrophoresis and mass spectrometry. The proteome analysis and data evaluation revealed that 70% of the 397 annotated proteins had been associated with different lysosome-related organelles in previous proteome studies.ConclusionWe provide the first comprehensive proteome map of T cell-derived secretory lysosomes with only minor contaminations by cytosolic, nuclear or other proteins. This information will be useful to more precisely address the activation-dependent maturation and the specific distribution of effector organelles and proteins in individual T or NK cell populations in future studies.

【 授权许可】

Unknown   
© Schmidt et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.