The additive effects of GS3 and qGL3 on rice grain length regulation revealed by genetic and transcriptome comparisons

BMC Plant Biology

Research Article

Ji Huang¹  Jianfei Wang¹  Xiuying Gao¹  Hongxia Lan¹  Hongsheng Zhang¹  Xiaojun Zhang² 

[1] State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, 210095, Nanjing, China;State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, 210095, Nanjing, China;College of Agronomy and Plant Protection, Qingdao Agricultural University, 266109, Qingdao, China;

关键词: Additive effect;    Grain length;    GS3;    qGL3;    Rice;    Transcriptome;    Brassinosteroid;   

DOI  :  10.1186/s12870-015-0515-4

 received in 2014-11-27, accepted in 2015-04-29,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundGrain length, as a critical trait for rice grain size and shape, has a great effect on grain yield and appearance quality. Although several grain size/shape genes have been cloned, the genetic interaction among these genes and the molecular mechanisms of grain size/shape architecture have not yet to be explored.ResultsTo investigate the genetic interaction between two major grain length loci of rice, GS3 and qGL3, we developed two near-isogenic lines (NILs), NIL-GS3 (GS3/qGL3) and NIL-qgl3 (gs3/qgl3), in the genetic background of 93–11 (gs3/qGL3) by conventional backcrossing and marker-assisted selection (MAS). Another NIL-GS3/qgl3 (GS3/qgl3) was developed by crossing NIL-GS3 with NIL-qgl3 and using MAS. By comparing the grain lengths of 93–11, NIL-GS3, NIL-qgl3 and NIL-GS3/qgl3, we investigated the effects of GS3, qGL3 and GS3 × qGL3 interaction on grain length based on two-way ANOVA. We found that GS3 and qGL3 had additive effects on rice grain length regulation. Comparative analysis of primary panicle transcriptomes in the four NILs revealed that the genes affected by GS3 and qGL3 partially overlapped, and both loci might be involved in brassinosteroid signaling.ConclusionOur data provide new information to better understand the rice grain length regulation mechanism and help rice breeders improve rice yield and appearance quality by molecular design breeding.

【 授权许可】

Unknown   
© Gao et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.