Testis-specific ATP synthase peripheral stalk subunits required for tissue-specific mitochondrial morphogenesis in Drosophila

BMC Cell Biology

Research Article

Yihharn Hwang¹  Conroy O. Field¹  Lauren E. Ivey¹  Kelsey E. Sheaffer¹  Eric M. Sawyer¹  Megan Bannon¹  E. Taylor Gunnell¹  Oshauna Morgan¹  Elizabeth C. Brunner¹  Nishita Suresh¹  Olivia Brown¹  M. Grace Gordon¹  Lindsay A. Regruto¹  Dennis Akrobetu¹  Karen G. Hales²  Cricket G. Wood³  Margaret T. Fuller³ 

[1] Department of Biology, Davidson College, Davidson, NC, USA;Department of Biology, Davidson College, Davidson, NC, USA;Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA, USA;Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA, USA;

关键词: Drosophila melanogaster;    Spermatogenesis;    Mitochondria;    ATP synthase;    Cristae;   

DOI  :  10.1186/s12860-017-0132-1

 received in 2016-11-18, accepted in 2017-03-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIn Drosophila early post-meiotic spermatids, mitochondria undergo dramatic shaping into the Nebenkern, a spherical body with complex internal structure that contains two interwrapped giant mitochondrial derivatives. The purpose of this study was to elucidate genetic and molecular mechanisms underlying the shaping of this structure.ResultsThe knotted onions (knon) gene encodes an unconventionally large testis-specific paralog of ATP synthase subunit d and is required for internal structure of the Nebenkern as well as its subsequent disassembly and elongation. Knon localizes to spermatid mitochondria and, when exogenously expressed in flight muscle, alters the ratio of ATP synthase complex dimers to monomers. By RNAi knockdown we uncovered mitochondrial shaping roles for other testis-expressed ATP synthase subunits.ConclusionsWe demonstrate the first known instance of a tissue-specific ATP synthase subunit affecting tissue-specific mitochondrial morphogenesis. Since ATP synthase dimerization is known to affect the degree of inner mitochondrial membrane curvature in other systems, the effect of Knon and other testis-specific paralogs of ATP synthase subunits may be to mediate differential membrane curvature within the Nebenkern.

【 授权许可】

CC BY   
© The Author(s). 2017