Prognostic significance of WT1 expression in soft tissue sarcoma

World Journal of Surgical Oncology

Research

Jeung Il Kim¹  Ahrong Kim²  Do Youn Park³  Chang Hun Lee³  Kyungbin Kim⁴  Jeong Hee Lee⁴  Eun Young Park⁵  Dong Hoon Shin⁶  Mee Young Sol⁶  Kyung Un Choi⁶  Jee Yeon Kim⁶  In Sook Lee⁷ 

[1] Department of Orthopedics, School of Medicine, Pusan National University, 626-870, Yangsan, Gyeongsangnam-do, Republic of Korea;Department of Pathology, Pusan National University Hospital, 602-739, Busan, Republic of Korea;Department of Pathology, Pusan National University Hospital, 602-739, Busan, Republic of Korea;Department of Pathology, School of Medicine, Pusan National University, 626-870, Yangsan, Gyeongsangnam-do, Republic of Korea;Department of Pathology, Pusan National University Yangsan Hospital, 626-770, Yangsan, Gyeongsangnam-do, Republic of Korea;Department of Pathology, School of Medicine, Pusan National University, 626-870, Yangsan, Gyeongsangnam-do, Republic of Korea;Department of Pathology, School of Medicine, Pusan National University, 626-870, Yangsan, Gyeongsangnam-do, Republic of Korea;Department of Pathology, Pusan National University Yangsan Hospital, 626-770, Yangsan, Gyeongsangnam-do, Republic of Korea;Department of Radiology, School of Medicine, Pusan National University, 626-870, Yangsan, Gyeongsangnam-do, Republic of Korea;

关键词: Soft Tissue Sarcoma;    Malignant Peripheral Nerve Sheath Tumor;    Soft Tissue Sarcoma;    Epithelioid Sarcoma;    Alveolar Soft Part Sarcoma;   

DOI  :  10.1186/1477-7819-12-214

 received in 2013-12-31, accepted in 2014-07-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSoft tissue sarcomas (STS) are rare. We evaluated the WT1 protein expression level in various types of STS and elucidated the value of WT1 as a prognostic factor and a possible therapeutic target.MethodsImmunohistochemical staining for WT1 was performed in 87 cases of STS using formalin-fixed, paraffin-embedded blocks. The correlation between WT1 expression and clinicopathological factors was analyzed. Survival analysis was conducted in 67 patients. We assessed the validity of WT1 immunohistochemistry as an index of WT1 protein expression using Western blot analysis.ResultsWT1 expression was noted in 47 cases (54.0%). Most rhabdomyosarcomas and malignant peripheral nerve sheath tumors showed WT1 expression (91.7% and 71.4%, respectively; P = 0.005). WT1 expression was related to higher FNCLCC histologic grade and AJCC tumor stage. In the group with high grade STS, strong WT1 expression was correlated with better survival (P = 0.025). The immunohistochemical results were correlated quantitatively with the staining score and the concentration of the Western blot band.ConclusionsThis study demonstrates that various types of STS show positive immunostaining for WT1 and that WT1 expression has a prognostic significance. So STS should be considered candidates for WT1 peptide--based immunotherapy.

【 授权许可】

Unknown   
© Kim et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.